CoraLite® Plus 750 Anti-Human CD25/IL-2RA (BC96)

• 

  FC experiment of human PBMCs using CL750-65096

  1X10^6 PHA treated human PBMCs were surface co-stained with FITC Anti-Human CD4 and 5 ul CoraLite® Plus 750 Anti-Human CD25 (CL750-65096, Clone:BC96) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CL750-65096

  1X10^6 day 3 PHA treated human PBMCs were surface stained with 5 ul CoraLite® Plus 750 Anti-Human CD25 (CL750-65096, Clone: BC96) (red) or Mouse IgG1 Isotype Control (blue). Unactivated PBMCs were surface stained with 5 ul CoraLite® Plus 750 Anti-Human CD25 (black). Cells were not fixed. Lymphocytes were gated.

CD25/IL-2RA Monoclonal Antibody for FC

Cat No. CL750-65096

Clone No.BC96

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Synonyms

CD25, BC96, IL2 RA, IL 2R subunit alpha, IL 2 receptor subunit alpha

---

Tested Applications

• Positive FC detected in: PHA treated human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in 100 μl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL750-65096 targets CD25/IL-2RA in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  N/A

• Full Name: interleukin 2 receptor, alpha
• Calculated Molecular Weight: 272 aa, 31 kDa
• GenBank Accession Number: BC156627
• Gene Symbol: IL2RA
• Gene ID (NCBI): 3559
• RRID: AB_3084844
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P01589
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells and a subset of human CD4+ memory T cells (PMID: 22585674).