CoraLite® Plus 647 Anti-Human CD25 (BC96)

CD25 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

BC96

Cat no : CL647-65096

Synonyms

IL-2 receptor subunit alpha, IL2 RA, IL 2R subunit alpha, IL 2 receptor subunit alpha, IL 2 RA

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Validation Data Gallery

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  FC experiment of human PBMCs using CL647-65096

  1x10^6 untreated (center) or PHA treated (3 days) human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD25 (CL647-65096, Clone:BC96) (right) or CoraLite® Plus 647 Mouse IgG1 Isotype Control (CL647-65124, Clone: MOPC-21) (left). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CL647-65096

  1x10^6 untreated (black dashed) or PHA treated (3 days) human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD25 (CL647-65096, Clone:BC96) (red) or CoraLite® Plus 647 Mouse IgG1 Isotype Control (CL647-65124, Clone: MOPC-21) (blue). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: PHA treated human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65096 targets CD25 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: interleukin 2 receptor, alpha
• Calculated Molecular Weight: 272 aa, 31 kDa
• GenBank Accession Number: BC156627
• Gene Symbol: IL2RA
• Gene ID (NCBI): 3559
• RRID: AB_2883679
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells and a subset of human CD4+ memory T cells (PMID: 22585674).