CD3 Monoclonal antibody

CD3 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b


human and More (1)







Cat no : 60181-1-Ig


CD3, CD3 epsilon, CD3E, CD3-epsilon, T3E, TCRE

Tested Applications

Positive WB detected inMOLT-4 cells, Jurkat cells, human PBMCs
Positive IHC detected inhuman tonsillitis tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue, Jurkat cells
Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy CD3 Ready-To-Use IHC Kit. CD3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®488, CoraLite®594 and CoraLite®647 conjugated versions of this antibody.

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:10000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

60181-1-Ig targets CD3 in WB, IHC, IF, FC applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD3 fusion protein Ag11797
Full Name CD3e molecule, epsilon (CD3-TCR complex)
Calculated molecular weight 207 aa, 23 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC049847
Gene symbol CD3E
Gene ID (NCBI) 916
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. This anti-CD3 is a monoclonal antibody directed against the epsilon chain of human CD3 molecule.


Product Specific Protocols
WB protocol for CD3 antibody 60181-1-IgDownload protocol
IHC protocol for CD3 antibody 60181-1-IgDownload protocol
IF protocol for CD3 antibody 60181-1-IgDownload protocol
FC protocol for CD3 antibody 60181-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Immunother Cancer

A PD-L2-based immune marker signature helps to predict survival in resected pancreatic ductal adenocarcinoma.

Authors - Yiyin Zhang

Oral Surg Oral Med Oral Pathol Oral Radiol

Regulatory T-cell expansion in oral and maxillofacial Langerhans cell histiocytosis.

Authors - Chuhan Zhang

Cell Death Discov

B7-H5 blockade enhances CD8+ T-cell-mediated antitumor immunity in colorectal cancer.

Authors - Jiayu Wang


Intrarenal macrophage infiltration induced by T cells is associated with podocyte injury in lupus nephritis patients.

Authors - R Ma

J Cell Mol Med

2-Deoxy-D-glucose impedes T cell-induced apoptosis of keratinocytes in oral lichen planus.

Authors - Fang Wang

J Cell Mol Med

T cell-derived exosomes induced macrophage inflammatory protein-1α/β drive the trafficking of CD8+ T cells in oral lichen planus.

Authors - Jing-Ya Yang