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CD3 Monoclonal antibody

CD3 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

3F3A1

Cat no : 60181-1-Ig

Synonyms

CD3, CD3 epsilon, CD3E, CD3-epsilon, T3E, TCRE



Tested Applications

Positive WB detected inMOLT-4 cells, Jurkat cells, human PBMCs
Positive IHC detected inhuman tonsillitis tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue, Jurkat cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:3200-1:12800
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

60181-1-Ig targets CD3 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD3 fusion protein Ag11797
Full Name CD3e molecule, epsilon (CD3-TCR complex)
Calculated molecular weight 207 aa, 23 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC049847
Gene symbol CD3E
Gene ID (NCBI) 916
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. This anti-CD3 is a monoclonal antibody directed against the epsilon chain of human CD3 molecule.

Protocols

Product Specific Protocols
WB protocol for CD3 antibody 60181-1-IgDownload protocol
IHC protocol for CD3 antibody 60181-1-IgDownload protocol
IF protocol for CD3 antibody 60181-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIF

J Med Virol

Combination of novel oncolytic herpesvirus with paclitaxel as an efficient strategy for breast cancer therapy

Authors - Xinyue Deng
humanIF

EBioMedicine

Patient-derived melanoma organoid models facilitate the assessment of immunotherapies

Authors - Lingling Ou
mouseIHC

Autophagy

Autophagy loss impedes cancer-associated fibroblast activation via downregulating proline biosynthesis.

Authors - Jingru Bai
ratIHC

Carbohydr Polym

Multifunctional chitin-based hollow nerve conduit for peripheral nerve regeneration and neuroma inhibition.

Authors - Xiaqing Yang
mouse,ratIHC

Pharmacol Res

CX-5461 is a potent immunosuppressant which inhibits T cell-mediated alloimmunity via p53-DUSP5.

Authors - Guopin Pan
humanIHC

Cancer Immunol Immunother

Novel roles of VAT1 expression in the immunosuppressive action of diffuse gliomas.

Authors - Pei Yang