CoraLite® Plus 488-conjugated CD3 Monoclonal antibody
CD3 Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG2b
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-60181
|Positive IF detected in
|human lung cancer tissue, human tonsillitis tissue
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
CL488-60181 targets CD3 in IF applications and shows reactivity with human samples.
|Host / Isotype
|Mouse / IgG2b
|CD3 fusion protein Ag11797
|CD3e molecule, epsilon (CD3-TCR complex)
|Calculated molecular weight
|207 aa, 23 kDa
|GenBank accession number
|Gene ID (NCBI)
|CoraLite® Plus 488 Fluorescent Dye
|Excitation/Emission maxima wavelengths
|493 nm / 522 nm
|Protein A purification
|PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
|Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. This anti-CD3 is a monoclonal antibody directed against the epsilon chain of human CD3 molecule. And the antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.