CoraLite®594-conjugated CD3 Monoclonal antibody

CD3 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

Publications

1

CloneNo.

3F3A1

Cat no : CL594-60181

Synonyms

CD3E, T3E, TCRE

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Validation Data Gallery

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  IF Staining of human tonsillitis using CL594-60181

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD3 antibody (CL594-60181, Clone: 3F3A1 ) at dilution of 1:400.

• 

  IF Staining of human tonsillitis using CL594-60181

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD3 antibody (CL594-60181, Clone: 3F3A1 ) at dilution of 1:400.

• 

  IF Staining of human tonsillitis using CL594-60181

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD3 antibody (CL594-60181, Clone: 3F3A1 ) at dilution of 1:400, PD-L1/CD274 (C-terminal) antibody (28076-1-AP, green).

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Tested Applications

• Positive IF detected in: human tonsillitis tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

CL594-60181 targets CD3 in IF applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD3 fusion protein Ag11797
• Full Name: CD3e molecule, epsilon (CD3-TCR complex)
• Calculated Molecular Weight: 207 aa, 23 kDa
• GenBank Accession Number: BC049847
• Gene Symbol: CD3E
• Gene ID (NCBI): 916
• RRID: AB_2883436
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. This anti-CD3 is a monoclonal antibody directed against the epsilon chain of human CD3 molecule. This antibody is CL594(Ex/Em 593 nm/614 nm) conjugated.

Publications

Species	Application	Title
human	IF	Cancer Lett; Spatial multimodal analysis revealed tertiary lymphoid structures as a risk stratification indicator in combined hepatocellular-cholangiocarcinoma; Authors - Xiaojie Gan