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CoraLite® Plus 488-conjugated CD59 Monoclonal antibody

CD59 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E3A1

Cat no : CL488-68222

Synonyms

16.3A5, 1F5, 1F5 antigen, CD59, CD59 glycoprotein, EJ16, EJ30, EL32, FLJ38134, FLJ92039, G344, HRF 20, HRF20, MAC inhibitory protein, MAC IP, MACIF, MEM43, MEM43 antigen, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, p18 20, Protectin

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using CoraLite® Plus 488-conjugated CD59 Monoclonal anti (CL488-68222)

    IF Staining of human placenta using CL488-68222

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CoraLite® Plus 488 CD59 antibody (CL488-68222, Clone: 1E3A1 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using CoraLite® Plus 488-conjugated CD59 Monoclonal anti (CL488-68222)

    IF Staining of human placenta using CL488-68222

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CoraLite® Plus 488 CD59 antibody (CL488-68222, Clone: 1E3A1 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of BxPC-3 cells using CoraLite® Plus 488-conjugated CD59 Monoclonal anti (CL488-68222)

    IF Staining of BxPC-3 using CL488-68222

    Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using CoraLite® Plus 488 CD59 antibody (CL488-68222, Clone: 1E3A1 ) at dilution of 1:200.

Tested Applications

Positive IF detected inhuman placenta tissue, BxPC-3 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68222 targets CD59 in IF applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD59 fusion protein Ag32834
Full Name CD59 molecule, complement regulatory protein
Calculated Molecular Weight 19 kDa
Observed Molecular Weight18-25 kDa
GenBank Accession NumberBC001506
Gene Symbol CD59
Gene ID (NCBI) 966
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD59, also named as MIC11, MIN1, MIN2, MIN3, MSK21, MIRL, MACIF, HRF20 and 1F5 antigen, is a cell surface molecule glycoprotein with MW 18-25 kDa. It acts as a determinant of proximal-distal cell identity. CD59 acts by binding to the C8 and/or C9 complements of the assembling membrane attack complex (MAC), thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. It is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CD59 antibody CL488-68222Download protocol
Standard Protocols
Click here to view our Standard Protocols