PE-Cyanine7 Anti-Mouse CD64 Rabbit Recombinant Antibody

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• PE-Cyanine7 Anti-Mouse CD64 Rabbit Recombinant Antibody (PY7-98218)

CD64 Uni-rAb^® Recombinant Antibody for FC

Cat No. PY7-98218

Clone No.241809A6

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

241809A6, Fcg1, Fc-gamma RI, Fcgr1, FcRI

• 

  FC experiment of mouse bone marrow cells using PY7-98218

  1x10^6 mouse bone marrow cells were surface stained with CoraLite® Plus 488 Anti-Mouse CD11b, and 0.1 ug PE-Cyanine7 Anti-Mouse CD64 Rabbit RecAb (PY7-98218, Clone: 241809A6) or PE-Cyanine7 Rabbit IgG Isotype Control Recombinant Antibody (PY7-98136, Clone: 240953C9). Cells were incubated with MonoZero™ Human/Mouse 3-in-1 blocking Reagent (PF00029) prior to staining. Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of mouse bone marrow cells using PY7-98218

  1x10^6 mouse bone marrow cells were surface stained with 0.1 ug PE-Cyanine7 Anti-Mouse CD64 Rabbit RecAb (PY7-98218, Clone: 241809A6) (red) or PE-Cyanine7 Rabbit IgG Isotype Control Recombinant Antibody (PY7-98136, Clone: 240953C9) (blue). Cells were incubated with MonoZero™ Human/Mouse 3-in-1 blocking Reagent (PF00029) prior to staining. Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: mouse bone marrow cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.1 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

PY7-98218 targets CD64 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2107

  Product name: Recombinant Mouse CD64 protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 25-297 aa of NM_010186.5

  Sequence: EVVNATKAVITLQPPWVSIFQKENVTLWCEGPHLPGDSSTQWFINGTAVQISTPSYSIPEASFQDSGEYRCQIGSSMPSDPVQLQIHNDWLLLQASRRVLTEGEPLALRCHGWKNKLVYNVVFYRNGKSFQFSSDSEVAILKTNLSHSGIYHCSGTGRHRYTSAGVSITVKELFTTPVLRASVSSPFPEGSLVTLNCETNLLLQRPGLQLHFSFYVGSKILEYRNTSSEYHIARAEREDAGFYWCEVATEDSSVLKRSPELELQVLGPQSSAP

• Full Name: Fc receptor, IgG, high affinity I
• Calculated Molecular Weight: 45 kDa
• GenBank Accession Number: NM_010186.5
• Gene Symbol: Fcgr1
• Gene ID (NCBI): 14129
• Conjugate: PE-Cyanine7 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 778 nm
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P26151
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Fcγ receptor comprise a multigene family of integral membrane glycoproteins that exhibit complex activation or inhibitory effects on cell functions after aggregation by complexed immunoglobulin G (IgG) (PMID: 17005690 ). CD64, also known as FcγRIA, is a high-affinity receptor for the Fc region of IgG. It is expressed by monocytes/macrophages, activated neutrophils, dendritic cells, and early myeloid cells (PMID: 23293080; 19642859; 7680917). CD64 functions in both innate and adaptive immune responses.