CoraLite® Plus 647 Anti-Mouse CD79a Rabbit Recombinant Antibody

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  FC experiment of mouse splenocytes using CL647-98129

  1x10^6 mouse splenocytes were intracellularly stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD79a Rabbit RecAb (CL647-98129, Clone: 241231F7) or 0.25 ug CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9). Cells were co-stained with CoraLite® Plus 488 Anti-Mouse CD45R (B220) (RA3-6B2) (CL488-65139, Clone: RA3-6B2). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of mouse splenocytes using CL647-98129

  1x10^6 mouse splenocytes were intracellularly stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD79a Rabbit RecAb (CL647-98129, Clone: 241231F7) (red) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CD79a Uni-rAb^TM Recombinant Antibody for FC (Intra)

Cat No. CL647-98129

Clone No.241231F7

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Synonyms

241231F7

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Tested Applications

• Positive FC (Intra) detected in: mouse splenocytes

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-98129 targets CD79a in FC (Intra) applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD79a fusion protein Eg1388
• Full Name: CD79A antigen (immunoglobulin-associated alpha)
• Calculated Molecular Weight: 25 kDa
• GenBank Accession Number: NM_007655.3
• Gene Symbol: Cd79a
• Gene ID (NCBI): 12518
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P11911
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD79a, also named as B-cell antigen receptor complex-associated protein alpha chain or MB-1 membrane glycoprotein, is a 226 amino acid protein, which contains one ITAM domain and one Ig-like C2-type (immunoglobulin-like) domain. CD79A is expressed in B cell and localizes in the cell membrane. CD79A is required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. CD79A is also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells.