FITC Plus Anti-Mouse CD80 (B7-1) (16-10A1)

CD80 (B7-1) Monoclonal Antibody for FC

Host / Isotype

Armenian Hamster / IgG

Reactivity

Mouse and More (1)

Applications

IF, FC

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

16-10A1

Cat no : FITC-65076

Synonyms

B7 1, B7.1, Cd28l, Cd80, CD80 (B7-1), CD80 antigen, Ly 53, Ly53, MIC17, TS/A 1



Tested Applications

Positive FC detected inmouse peritoneal macrophages, Balb/c mouse peritoneal macrophages

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

FITC-65076 targets CD80 (B7-1) in IF, FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Cited Reactivitymouse
Host / Isotype Armenian Hamster / IgG
Class Monoclonal
Type Antibody
Immunogen CHO cell line transfected with mouse B7 (CD80)
Full Name CD80 antigen
GenBank Accession NumberBC131959
Gene Symbol Cd80
Gene ID (NCBI) 12519
RRIDAB_2883762
Conjugate FITC Plus Fluorescent Dye
Excitation/Emission Maxima Wavelengths495 nm / 524 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)

Protocols

Product Specific Protocols
FC protocol for FITC Plus CD80 (B7-1) antibody FITC-65076Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseFC

ACS Appl Mater Interfaces

Combined Chemo-Immuno-Photothermal Therapy for Effective Cancer Treatment via an All-in-One and One-for-All Nanoplatform

Authors - Xianglong Yu
mouseFC

ACS Appl Mater Interfaces

Intelligent Nanoplatform Integrating Macrophage and Cancer Cell Membrane for Synergistic Chemodynamic/Immunotherapy/Photothermal Therapy of Breast Cancer

Authors - Shichao Zhang
mouseIF

Cell Rep

Mechanotransduction in response to ECM stiffening impairs cGAS immune signaling in tumor cells

Authors - Yingqi Liu
mouseFC

Neurobiol Dis

Inhibitory effects of xanthine oxidase inhibitor, topiroxostat, on development of neuropathy in db/db mice.

Authors - Kazuhisa Takahashi
FC

Nat Commun

CD36-mediated metabolic crosstalk between tumor cells and macrophages affects liver metastasis

Authors - Ping Yang
mouse

Mol Immunol

Paeonol interferes with lupus nephritis by regulating M1/M2 polarization of macrophages

Authors - Yuzhen Niu