FITC Plus Anti-Mouse CD80 (16-10A1)

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  FC experiment of Balb/c mouse peritoneal macrophages using FITC-65076

  1x10^6 Balb/c mouse peritoneal macrophages were surface stained with 0.5 ug FITC Plus Anti-Mouse CD80 (B7-1) (16-10A1) (FITC-65076, Clone: 16-10A1) (red), or 0.5 ug FITC Armenian Hamster IgG Isotype Control (PIP) (FITC-65210, Clone: PIP) (blue). Cells were not fixed.

CD80 Monoclonal Antibody for FC

Cat No. FITC-65076

Clone No.16-10A1

Publications(13)

Host / Isotype

Armenian Hamster / IgG

Reactivity

mouse

Applications

IF, FC

Synonyms

Ly53, Ly 53, CD80 antigen, Cd28l, B7.1

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Tested Applications

• Positive FC detected in: Balb/c mouse peritoneal macrophages

Recommended dilution

• Flow Cytometry (FC): FC : 0.5 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 2 publications below
• FC: See 11 publications below

Product Information

FITC-65076 targets CD80 in IF, FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: mouse
• Host / Isotype: Armenian Hamster / IgG
• Class: Monoclonal
• Type: Antibody
• Immunogen: CHO cell line transfected with mouse B7 (CD80)
• Full Name: CD80 antigen
• GenBank Accession Number: BC131959
• Gene Symbol: CD80
• Gene ID (NCBI): 12519
• RRID: AB_2883762
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: Q00609
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)

Publications

Species	Application	Title
mouse	FC	ACS Appl Mater Interfaces; Combined Chemo-Immuno-Photothermal Therapy for Effective Cancer Treatment via an All-in-One and One-for-All Nanoplatform; Authors - Xianglong Yu
mouse	FC	ACS Appl Mater Interfaces; Intelligent Nanoplatform Integrating Macrophage and Cancer Cell Membrane for Synergistic Chemodynamic/Immunotherapy/Photothermal Therapy of Breast Cancer; Authors - Shichao Zhang
mouse	IF	Cell Rep; Mechanotransduction in response to ECM stiffening impairs cGAS immune signaling in tumor cells; Authors - Yingqi Liu
mouse	FC	Neurobiol Dis; Inhibitory effects of xanthine oxidase inhibitor, topiroxostat, on development of neuropathy in db/db mice.; Authors - Kazuhisa Takahashi
	FC	Nat Commun; CD36-mediated metabolic crosstalk between tumor cells and macrophages affects liver metastasis; Authors - Ping Yang
mouse		Mol Immunol; Paeonol interferes with lupus nephritis by regulating M1/M2 polarization of macrophages; Authors - Yuzhen Niu