CoraLite® Plus 488 Anti-Mouse CD80 (B7-1) (16-10A1)

CD80 (B7-1) Monoclonal Antibody for FC

Host / Isotype

Armenian Hamster / IgG

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

16-10A1

Cat no : CL488-65076

Synonyms

B7 1, B7.1, Cd28l, Cd80, CD80 (B7-1), CD80 antigen, Ly 53, Ly53, MIC17, TS/A 1

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Validation Data Gallery

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  FC experiment of BALB/C mouse peritoneal macrophages using CL488-65076

  1X10^6 BALB/C mouse peritoneal macrophages were surface stained with 0.5 ug CoraLite® Plus 488 Anti-Mouse CD80 (B7-1) (CL488-65076, Clone:16-10A1), or unstained. Cells were not fixed.

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Tested Applications

• Positive FC detected in: BALB/C mouse peritoneal macrophages

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65076 targets CD80 (B7-1) in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Armenian Hamster / IgG
• Class: Monoclonal
• Type: Antibody
• Immunogen: CHO cell line transfected with mouse B7 (CD80)
• Full Name: CD80 antigen
• GenBank Accession Number: BC131959
• Gene Symbol: Cd80
• Gene ID (NCBI): 12519
• RRID: AB_2919255
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)