Biotin Anti-Human CD83 (HB15e) Mouse IgG2a Recombinant Antibody

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  FC experiment of human monocyte-derived mature dendritic cells using Biotin-65581

  1x10^6 human monocyte-derived mature dendritic cells were surface stained with 0.25 ug Biotin Anti-Human CD83 (HB15e) Mouse IgG2a RecAb (Biotin-65581, Clone: HB15e) (red) or unstained (blue), and CoraLite®488-conjugated streptavidin. Cells were not fixed.

CD83 Uni-rAb^TM Recombinant Antibody for FC

Cat No. Biotin-65581

Clone No.HB15e

Host / Isotype

Mouse / IgG2a

Reactivity

human, non-human primates

Applications

FC

Synonyms

B cell activation protein, B-cell activation protein, BL11, CD83 antigen, CD83 molecule

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Tested Applications

• Positive FC detected in: human monocyte-derived mature dendritic cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-65581 targets CD83 in FC applications and shows reactivity with human, non-human primates samples.

• Tested Reactivity: human, non-human primates
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: Human CD83-transfected Cos cells
• Full Name: CD83 molecule
• Calculated Molecular Weight: 205 aa, 23 kDa
• GenBank Accession Number: BC030830
• Gene Symbol: CD83
• Gene ID (NCBI): 9308
• ENSEMBL Gene ID: ENSG00000112149
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD83 is a member of the immunoglobulin (Ig) superfamily, consisting of an extracellular V-type Ig-like domain, a transmembrane domain, and a cytoplasmic tail. CD83 is one of the most prominent markers for fully matured dendritic cells (DCs) (PMID: 17334966). It is also found on the surface of other activated hematopoietic cells, including lymphocytes, monocytes, macrophages, and neutrophils (PMID: 31231400; 17334966). CD83 regulates the maturation, activation, and homeostasis of numerous immune cells (PMID: 31231400; 32362900). CD83 can also expressed as a soluble form. Soluble CD83 can bind to DCs and inhibit their maturation (PMID: 17334966; 12403928).