FC experiment of human monocyte-derived mature dendritic cells using CL647-65581
1x10^6 human monocyte-derived mature dendritic cells were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD83 (HB15e) Mouse IgG2a Recombinant Antibody (CL647-65581, Clone: HB15e) (red) or CoraLite® Plus 647 Mouse IgG2a Isotype Control (C1.18.4) (CL647-65208, Clone: C1.18.4) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
1x10^6 human monocyte-derived mature dendritic cells were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD83 (HB15e) Mouse IgG2a Recombinant Antibody (CL647-65581, Clone: HB15e) (red) or CoraLite® Plus 647 Mouse IgG2a Isotype Control (C1.18.4) (CL647-65208, Clone: C1.18.4) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
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This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.
Product Information
CL647-65581 targets CD83 in FC applications and shows reactivity with human, non-human primates samples.
FC experiment of human monocyte-derived mature dendritic cells using CL647-65581
1x10^6 human monocyte-derived mature dendritic cells were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD83 (HB15e) Mouse IgG2a Recombinant Antibody (CL647-65581, Clone: HB15e) (red) or CoraLite® Plus 647 Mouse IgG2a Isotype Control (C1.18.4) (CL647-65208, Clone: C1.18.4) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
CD83 Recombinant antibody
Catalog Number
CL647-65581
Citations
-
Dilutions
Applications
FC
Reactivity
human, non-human primates
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
