CoraLite® Plus 750 Anti-Human CD83 (HB15e) Mouse IgG2a Recombinant Antibody

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  FC experiment of human monocyte-derived mature dendritic cells using CL750-65581

  1x10^6 human monocyte-derived mature dendritic cells were surface stained with 5 ul CoraLite® Plus 750 Anti-Human CD83 (HB15e) Mouse IgG2a Recombinant Antibody (CL750-65581, Clone: HB15e) (red) or CoraLite® Plus 750 Mouse IgG2a Isotype Control (C1.18.4) (CL750-65208, Clone: C1.18.4) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

CD83 Uni-rAb^TM Recombinant Antibody for FC

Cat No. CL750-65581

Clone No.HB15e

Host / Isotype

Mouse / IgG2a

Reactivity

human, non-human primates

Applications

FC

Synonyms

B cell activation protein, B-cell activation protein, BL11, CD83 antigen, CD83 molecule

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Tested Applications

• Positive FC detected in: human monocyte-derived mature dendritic cells

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL750-65581 targets CD83 in FC applications and shows reactivity with human, non-human primates samples.

• Tested Reactivity: human, non-human primates
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: Human CD83-transfected Cos cells
• Full Name: CD83 molecule
• Calculated Molecular Weight: 205 aa, 23 kDa
• GenBank Accession Number: BC030830
• Gene Symbol: CD83
• Gene ID (NCBI): 9308
• ENSEMBL Gene ID: ENSG00000112149
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD83 is a member of the immunoglobulin (Ig) superfamily, consisting of an extracellular V-type Ig-like domain, a transmembrane domain, and a cytoplasmic tail. CD83 is one of the most prominent markers for fully matured dendritic cells (DCs) (PMID: 17334966). It is also found on the surface of other activated hematopoietic cells, including lymphocytes, monocytes, macrophages, and neutrophils (PMID: 31231400; 17334966). CD83 regulates the maturation, activation, and homeostasis of numerous immune cells (PMID: 31231400; 32362900). CD83 can also expressed as a soluble form. Soluble CD83 can bind to DCs and inhibit their maturation (PMID: 17334966; 12403928).