FcZero-rAb® APC Anti-Human CD99 Rabbit Recombinant Antibody

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• FcZero-rAb® APC Anti-Human CD99 Rabbit Recombinant Antibody (APC-FcA98174)

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

CD99 FcZero-rAb^® Recombinant Antibody for FC

Cat No. APC-FcA98174 Copied!

Clone No. 241737H1

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

12E7, CD99 antigen, CD99 molecule, E2 antigen, MIC2

• 

  FC experiment of human PBMCs using APC-FcA98174

  1x10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD99 Rabbit RecAb (APC-FcA98174, Clone: 241737H1) or APC Rabbit IgG Isotype Control RecAb (APC-FcA98136, Clone: 240953C9). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of human PBMCs using APC-FcA98174

  1x10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD99 Rabbit RecAb (APC-FcA98174, Clone: 241737H1) (red) or APC Rabbit IgG Isotype Control RecAb (APC-FcA98136, Clone: 240953C9) (blue). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

APC-FcA98174 targets CD99 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant Protein

• Full Name: CD99 molecule
• Calculated Molecular Weight: 19 kDa, 16 kDa, 17 kDa
• GenBank Accession Number: BC021620
• Gene Symbol: CD99
• Gene ID (NCBI): 4267
• RRID: AB_3746578
• Conjugate: APC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 650 nm / 660 nm
• Excitation Laser: Red Laser (633 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P14209
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD99, also known as MIC2, is a heavily O-glycosylated transmembrane protein involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. CD99 is broadly distributed on many cell types, with particularly strong expression on human cortical thymocytes, Ewing's sarcoma cells and peripheral primitive neuroectodermal tumors (PMID: 9794396; 16984917). In normal cells, CD99 has been functionally implicated in cell adhesion, migration, apoptosis, differentiation, activation, and proliferation of lymphocytes and monocyte extravasation and transport of several transmembrane proteins (PMID: 16984917).