FcZero-rAb® Biotin Anti-Human CD99 Rabbit Recombinant Antibody

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• FcZero-rAb® Biotin Anti-Human CD99 Rabbit Recombinant Antibody (Biotin-FcA98174)

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

CD99 FcZero-rAb^® Recombinant Antibody for FC, ELISA

Cat No. Biotin-FcA98174 Copied!

Clone No. 241737H1

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, ELISA

Synonyms

12E7, CD99 antigen, CD99 molecule, E2 antigen, MIC2

• 

  FC experiment of human PBMCs using Biotin-FcA98174

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD99 Rabbit RecAb (Biotin-FcA98174, Clone:241737H1) or FcZero-rAb™ Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9), and Streptavidin-PE Conjugate (PE-PF00030). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of human PBMCs using Biotin-FcA98174

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD99 Rabbit RecAb (Biotin-FcA98174, Clone: 241737H1) (red) or FcZero-rAb™ Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9) (blue), and Streptavidin-PE Conjugate (PE-PF00030). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-FcA98174 targets CD99 in FC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant Protein

• Full Name: CD99 molecule
• Calculated Molecular Weight: 19 kDa, 16 kDa, 17 kDa
• GenBank Accession Number: BC021620
• Gene Symbol: CD99
• Gene ID (NCBI): 4267
• RRID: AB_3746955
• Conjugate: Biotin
• Excitation/Emission Maxima Wavelengths: -
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P14209
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD99, also known as MIC2, is a heavily O-glycosylated transmembrane protein involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. CD99 is broadly distributed on many cell types, with particularly strong expression on human cortical thymocytes, Ewing's sarcoma cells and peripheral primitive neuroectodermal tumors (PMID: 9794396; 16984917). In normal cells, CD99 has been functionally implicated in cell adhesion, migration, apoptosis, differentiation, activation, and proliferation of lymphocytes and monocyte extravasation and transport of several transmembrane proteins (PMID: 16984917).