• Zero Fc receptor binding
  • Site-specific conjugation
  • One universal isotype

FcZero-rAb™ PE Anti-Human CD99 Rabbit Recombinant Antibody

CD99 FcZero-rAbTM Recombinant Antibody for FC
Cat No. PE-FcA98174
Clone No.241737H1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

12E7, CD99 antigen, CD99 molecule, E2 antigen, MIC2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  PE
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 5 ul per 10^6 cells in a 100 µl suspension
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-FcA98174 targets CD99 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein Predict reactive species
Full Name CD99 molecule
Calculated Molecular Weight 19 kDa, 16 kDa, 17 kDa
GenBank Accession NumberBC021620
Gene Symbol CD99
Gene ID (NCBI) 4267
Conjugate PE Fluorescent Dye
Excitation/Emission Maxima Wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD99, also known as MIC2, is a heavily O-glycosylated transmembrane protein involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. CD99 is broadly distributed on many cell types, with particularly strong expression on human cortical thymocytes, Ewing's sarcoma cells and peripheral primitive neuroectodermal tumors (PMID: 9794396; 16984917). In normal cells, CD99 has been functionally implicated in cell adhesion, migration, apoptosis, differentiation, activation, and proliferation of lymphocytes and monocyte extravasation and transport of several transmembrane proteins (PMID: 16984917).

Protocols

Product Specific Protocols
FC protocol for PE CD99 antibody PE-FcA98174Download protocol
Standard Protocols
Click here to view our Standard Protocols
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