CDC27; APC3 Monoclonal antibody

CDC27; APC3 Monoclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

CloneNo.

2F10G2

Cat no : 67239-1-Ig

Synonyms

ANAPC3, APC3, CDC27, CDC27 homolog, CDC27; APC3, CDC27Hs, D0S1430E, D17S978E, H NUC, HNUC



Tested Applications

Positive WB detected inJurkat cells, HSC-T6 cells, 4T1 cells, HEK-293 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells
Positive FC detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

67239-1-Ig targets CDC27; APC3 in WB, IHC, IF, FC,ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CDC27; APC3 fusion protein Ag25588
Full Name cell division cycle 27 homolog (S. cerevisiae)
Calculated molecular weight 92 kDa
Observed molecular weight 90-100 kDa
GenBank accession numberBC011656
Gene symbol CDC27
Gene ID (NCBI) 996
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

CDC27/APC3 is a component of the anaphase-promoting complex (APC/cyclosome), which is composed of eight subunits and highly conserved in eukaryotic cells. The APC/cyclosome complex acts as a cell cycle-regulated E3 ubiquitin ligase which mediates ubiquitination and subsequent degradation of target proteins, and lead to the progression control through mitosis and the G1 phase of the cell cycle.

Protocols

Product Specific Protocols
WB protocol for CDC27; APC3 antibody 67239-1-IgDownload protocol
IHC protocol for CDC27; APC3 antibody 67239-1-IgDownload protocol
IF protocol for CDC27; APC3 antibody 67239-1-IgDownload protocol
FC protocol for CDC27; APC3 antibody 67239-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols