CEP97 Polyclonal antibody, PBS Only

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• CEP97 Polyclonal antibody, PBS Only (22050-1-PBS)

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  WB analysis of HL-60 using 22050-1-AP (same clone as 22050-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 22050-1-AP (CEP97 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 22050-1-AP (same clone as 22050-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 22050-1-AP (CEP97 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 22050-1-AP (same clone as 22050-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22050-1-AP (CEP97 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 22050-1-AP (same clone as 22050-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 22050-1-AP (CEP97 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 22050-1-AP (same clone as 22050-1-PBS)

  IP result of anti-CEP97 (IP:22050-1-AP, 4ug; Detection:22050-1-AP 1:1000) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 22050-1-AP (same clone as 22050-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 22050-1-AP (CEP97 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 22050-1-AP (same clone as 22050-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 22050-1-AP (CEP97 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 22050-1-AP (same clone as 22050-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 22050-1-AP (CEP97 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 22050-1-AP (same clone as 22050-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 22050-1-AP (CEP97 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

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  IF Staining of MDCK using 22050-1-AP (same clone as 22050-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CEP97 antibody (22050-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

CEP97 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 22050-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, canine

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

centrosomal protein 97kDa, Centrosomal protein of 97 kDa, Leucine-rich repeat and IQ domain-containing protein 2, LRRIQ2

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Product Information

22050-1-PBS targets CEP97 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, canine samples.

• Tested Reactivity: human, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16995

  Product name: Recombinant human CEP97 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 506-865 aa of BC041085

  Sequence: ESTEQKQSDIKKPENTQPENKETISQATSEKLPMILTQRSVALGQDKVALQKLNDAATKLQACWRGFYARNYNPQAKDVRYEIRLRRMQEHIVCLTDEIRRLRKERDEERIKKFVQEEAFRFLWNQVRSLQVWQQTVDQRLSSWHTDVPPISSTLVPSKHPLFTQSQESSCDQNADWFIASDVAPQEKSLPEFPDSGFHSSLTEQVHSLQHSLDFEKSSTEGSESSIMGNSIDTVRYGKESDLGDVSEEHGEWNKESSNNEQDNSLLEQYLTSVQQLEDADERTNFDTETRDSKLHIACFPVQLDTLSDGASVDESHGISPPLQGEISQTQENSKLNAEVQGQQPECDSTFQLLHVGVTV

• Full Name: centrosomal protein 97kDa
• Calculated Molecular Weight: 865 aa, 97 kDa
• Observed Molecular Weight: 130-140 kDa
• GenBank Accession Number: BC041085
• Gene Symbol: CEP97
• Gene ID (NCBI): 79598
• RRID: AB_11182378
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IW35
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CEP97, also named as LRRIQ2, contains one IQ domain, eight LRR (leucine-rich) repeats and one LRRCT domain. It collaborates with CEP110, being involved in the suppression of a cilia assembly program. CEP97 is required for correct spindle formation and has a role in cytokinesis. It is required for recruitment of CEP110 to the centrosome. This protein has a calculated MW ~97 kDa, although the protein migrates at a molecular weight of approximate 140 kDa. The protein is highly acidic, with a pI ~4.7, providing a potential explanation for its reduced mobility in SDS-PAGE. [PMID: 17719545]. This is a rabbit polyclonal antibody raised against the C terminus of human CEP97.