Tested Applications
| Positive WB detected in | HL-60 cells, HeLa cells, human heart tissue |
| Positive IF/ICC detected in | hTERT-RPE1 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12794-1-AP targets Centrin 1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3529 Product name: Recombinant human CETN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-172 aa of BC029515 Sequence: MASGFKKPSAASTGQKRKVAPKPELTEDQKQEVREAFDLFDVDGSGTIDAKELKVAMRALGFEPRKEEMKKMISEVDREGTGKISFNDFLAVMTQKMSEKDTKEEILKAFRLFDDDETGKISFKNLKRVANELGENLTDEELQEMIDEADRDGDGEVNEEEFLRIMKKTSLY Predict reactive species |
| Full Name | centrin, EF-hand protein, 1 |
| Calculated Molecular Weight | 172 aa, 20 kDa |
| Observed Molecular Weight | 20 kDa |
| GenBank Accession Number | BC029515 |
| Gene Symbol | Centrin 1 |
| Gene ID (NCBI) | 1068 |
| RRID | AB_2077371 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q12798 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
EF-hand type Ca2+-binding proteins consists of several family members, including Centrin-1, Centrin-2 and Centrin-3. The Centrin proteins are ubiquitously expressed cytoskeletal components that show increased expression during cell differentiation. Centrin-1 plays important roles in the determination of centrosome position and segregation, and in the process of microtubule severing. Centrin-1 is localized to the centrosome of interphase cells, and redistributes to the region of the spindle poles during mitosis, reflecting the dynamic behavior of the centrosome during the cell cycle.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Centrin 1 antibody 12794-1-AP | Download protocol |
| WB protocol for Centrin 1 antibody 12794-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
12794-1-AP (anti-γ-tubulin) has 56 citations across journals including Nature Communications, Cell Research, Nature Cell Biology, Neuron, Developmental Cell, EMBO Journal, Journal of Cell Biology, and eLife. Research fields span centriole biogenesis, ciliogenesis, centrosome duplication, mitotic spindle dynamics, neuronal migration, ciliopathies, male infertility, photoreceptor biology, and cancer. Applications are predominantly immunofluorescence and Western blot in human, mouse, and zebrafish models.
| Species | Application | Title |
|---|---|---|
Cell Res Misfolded Gβ is recruited to cytoplasmic dynein by Nudel for efficient clearance. | ||
Nat Cell Biol The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis. | ||
Protein Cell O-GlcNAc transferase regulates centriole behavior and intraflagellar transport to promote ciliogenesis. | ||
Nat Commun Uncovering structural themes across cilia microtubule inner proteins with implications for human cilia function |
Reviews
What customers say
The single review for this product reports successful immunofluorescence staining of ciliated human fibroblasts at a 1:100 dilution, with the user describing the staining as "nice." The reviewer rated the product 4 out of 5 stars. Overall, the feedback is positive, indicating reliable performance for IF applications in human cell lines.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Julie (Verified Customer) (02-14-2019) | Nice staining of ciliated human fibroblasts.
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