Centrin 1 Polyclonal antibody

Centrin 1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

39

Reviews (1)

Cat no : 12794-1-AP

Synonyms

CETN1, CETN, Centrin-1, CEN1, Caltractin isoform 2

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Validation Data Gallery

• 

  WB analysis of HL-60 using 12794-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 12794-1-AP (Centrin 1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 12794-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 12794-1-AP (Centrin 1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 12794-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12794-1-AP (Centrin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of hTERT-RPE1 using 12794-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using Centrin 1 antibody (12794-1-AP) at dilution of 1:50 and Cy3-conjugated Affinipure Goat Anti-Rabbit IgG(H+L), CoraLite®488 ARL13B antibody (CL488-17711, green), SCLT1 antibody (14875-1-AP, Magenta).

• 

  IF Staining of HeLa using 12794-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Centrin 1 antibody (12794-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of hTERT-RPE1 using 12794-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using Centrin 1 antibody (12794-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of hTERT-RPE1 using 12794-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using Centrin 1 antibody (12794-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

• 

  IF Staining of HeLa using 12794-1-AP

  Immunofluorescent analysis of Hela cells, using CETN1 antibody 12794-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HL-60 cells, human heart tissue
• Positive IF/ICC detected in: hTERT-RPE1 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IF: See 32 publications below

Product Information

12794-1-AP targets Centrin 1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Centrin 1 fusion protein Ag3529
• Full Name: centrin, EF-hand protein, 1
• Calculated Molecular Weight: 172 aa, 20 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC029515
• Gene Symbol: Centrin 1
• Gene ID (NCBI): 1068
• RRID: AB_2077371
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EF-hand type Ca2+-binding proteins consists of several family members, including Centrin-1, Centrin-2 and Centrin-3. The Centrin proteins are ubiquitously expressed cytoskeletal components that show increased expression during cell differentiation. Centrin-1 plays important roles in the determination of centrosome position and segregation, and in the process of microtubule severing. Centrin-1 is localized to the centrosome of interphase cells, and redistributes to the region of the spindle poles during mitosis, reflecting the dynamic behavior of the centrosome during the cell cycle.

Publications

Species	Application	Title
human, mouse	WB,IF	Nat Cell Biol; The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis.; Authors - Huijie Zhao
human		Neuron; Posterior Neocortex-Specific Regulation of Neuronal Migration by CEP85L Identifies Maternal Centriole-Dependent Activation of CDK5.; Authors - Andrew Kodani
human	IF	Cell Res; NudC regulates actin dynamics and ciliogenesis by stabilizing cofilin 1.; Authors - Cheng Zhang
mouse	IF	Nat Commun; Cytoplasmic E2f4 forms organizing centres for initiation of centriole amplification during multiciliogenesis.; Authors - Munemasa Mori
zebrafish	IF	Nucleic Acids Res; Resting cells rely on the DNA helicase component MCM2 to build cilia.; Authors - Teresa Casar Tena
human	IF	Nat Commun; DNA replication licensing factor Cdc6 and Plk4 kinase antagonistically regulate centrosome duplication via Sas-6.; Authors - Xiaowei Xu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Julie (Verified Customer) (02-14-2019)

Nice staining of ciliated human fibroblasts.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: human fibroblasts

Centrin 1 Antibody Immunofluorescence, validation (1:100 dilution) in human fibroblasts (Cat no:12794-1-AP)

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