CoraLite® Plus 488-conjugated Properdin/PFC Monoclonal antibody

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  WB analysis using CL488-66788

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-66788 (CFP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Properdin/PFC Monoclonal Antibody for WB

Cat No. CL488-66788

Clone No.1D5A9

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig

Applications

WB

Synonyms

CFP, PFC, complement factor properdin, Complement factor P, BFD

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Tested Applications

• Positive WB detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66788 targets Properdin/PFC in WB applications and shows reactivity with human, rat, pig samples.

• Tested Reactivity: human, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Properdin/PFC fusion protein Ag9798
• Full Name: complement factor properdin
• Calculated Molecular Weight: 469 aa, 51 kDa
• Observed Molecular Weight: 50 kDa, 100 kDa, 150 kDa
• GenBank Accession Number: BC015756
• Gene Symbol: CFP
• Gene ID (NCBI): 5199
• RRID: AB_3084274
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P27918
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CFP, also named as Properdin or CFP, is a 469 amino acid protein, which contains 7 TSP type-1 domains. CFP as secreted protein is a positive regulator of the alternate pathway of complement. It binds to and stabilizes the C3- and C5-convertase enzyme complexes. Properdin deficiency poses a significant risk for severe meningococcal infections (PMID: 22229731). Properdin may be novel biomarker for future risk of type 2 diabetes (PMID: 22338105). In plasma, properdin exists as dimers, trimers or tetramers.