CIB1 Monoclonal antibody

CIB1 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, bovine

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1D2C12

Cat no : 67901-1-Ig

Synonyms

Calmyrin, CIB, CIB1, CIBP, DNA PKcs interacting protein, Kinase interacting protein, KIP, KIP1, PRKDCIP, SIP2 28, SNK interacting protein 2 28

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Validation Data Gallery

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  WB analysis using 67901-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67901-1-Ig (CIB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67901-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67901-1-Ig (CIB1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67901-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67901-1-Ig (CIB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: LNCaP cells, Hela cells, K-562 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, Human milk
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67901-1-Ig targets CIB1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, bovine samples.

• Tested Reactivity: Human, mouse, rat, bovine
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CIB1 fusion protein Ag31174
• Full Name: calcium and integrin binding 1 (calmyrin)
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC000846
• Gene Symbol: CIB1
• Gene ID (NCBI): 10519
• RRID: AB_2918657
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.