CoraLite® Plus 488-conjugated CIB1 Monoclonal antibody

CIB1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, bovine

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D2C12

Cat no : CL488-67901

Synonyms

Calmyrin, CIB, CIB1, CIBP, DNA PKcs interacting protein, Kinase interacting protein, KIP, KIP1, PRKDCIP, SIP2 28, SNK interacting protein 2 28

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Validation Data Gallery

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  FC experiment of Jurkat using CL488-67901

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CIB1 (CL488-67901, Clone:1D2C12) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC detected in: Jurkat cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67901 targets CIB1 in FC (Intra) applications and shows reactivity with Human, mouse, rat, bovine samples.

• Tested Reactivity: Human, mouse, rat, bovine
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CIB1 fusion protein Ag31174
• Full Name: calcium and integrin binding 1 (calmyrin)
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC000846
• Gene Symbol: CIB1
• Gene ID (NCBI): 10519
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C