- Featured Product
- KD/KO Validated
CLTC Polyclonal antibody
CLTC Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 26523-1-AP
Validation Data Gallery
|Positive WB detected in
|HeLa cells, HL-60 cells, pig brain tissue, Raji cells, mouse brain tissue, rat brain tissue
|Positive IP detected in
|Positive IHC detected in
|human breast cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|HepG2 cells, HeLa cells
|Positive FC detected in
|Western Blot (WB)
|WB : 1:2000-1:10000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:250-1:1000
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
26523-1-AP targets CLTC in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, pig, mouse, rat samples.
|human, pig, mouse, rat
|human, mouse, bovine
|Host / Isotype
|Rabbit / IgG
|CLTC fusion protein Ag25041
|clathrin, heavy chain (Hc)
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (CLTC) (160-190 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The heavy chain provides the structural backbone of the clathrin lattice. (PMID: 9133677)
|Product Specific Protocols
|WB protocol for CLTC antibody 26523-1-AP
|IHC protocol for CLTC antibody 26523-1-AP
|IF protocol for CLTC antibody 26523-1-AP
|IP protocol for CLTC antibody 26523-1-AP
|FC protocol for CLTC antibody 26523-1-AP
|Click here to view our Standard Protocols
Emerg Microbes Infect
CD1d facilitates African swine fever virus entry into the host cells via clathrin-mediated endocytosis
AP2M1 mediates autophagy-induced CLDN2 (claudin 2) degradation through endocytosis and interaction with LC3 and reduces intestinal epithelial tight junction permeability.
Br J Pharmacol
A glycine-serine-alanine-rich peptide ameliorates Alzheimer-type neurodegeneration
Aging (Albany NY)
The interaction protein of SORBS2 in myocardial tissue to find out the pathogenic mechanism of LVNC disease.
Invasion of Mycoplasma bovis into bovine synovial cells utilizing the clathrin-dependent endocytosis pathway.
A genome-wide transposon mutagenesis screening identifies LppB as a key factor associated with Mycoplasma bovis colonization and invasion into host cells