CLTC Polyclonal antibody

• Featured Product
• KD/KO Validated

CLTC Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, pig, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 26523-1-AP

Synonyms

CHC, CHC17, Clathrin heavy chain 1, clathrin, heavy chain (Hc), CLH 17, CLH17, CLTC, CLTCL2, Hc, KIAA0034

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Validation Data Gallery

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  WB analysis of HeLa using 26523-1-AP

  WB result of CLTC antibody (26523-1-AP; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLTC transfected HeLa cells.

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  WB analysis using 26523-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26523-1-AP (CLTC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig brain using 26523-1-AP

  pig brain tissue were subjected to SDS PAGE followed by western blot with 26523-1-AP (CLTC Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 26523-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 26523-1-AP (CLTC Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 26523-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 26523-1-AP (CLTC Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 26523-1-AP

  IP Result of anti-CLTC (IP:26523-1-AP, 4ug; Detection:26523-1-AP 1:500) with HeLa cells lysate 2500ug.

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  IHC staining of human breast cancer using 26523-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26523-1-AP (CLTC Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 26523-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26523-1-AP (CLTC Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 26523-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26523-1-AP (CLTC Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 26523-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26523-1-AP (CLTC Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 26523-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 26523-1-AP (CLTC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HL-60 cells, pig brain tissue, Raji cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human breast cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 3 publications below

Product Information

The immunogen of 26523-1-AP is CLTC Fusion Protein expressed in E. coli.

• Tested Reactivity: human, pig, mouse, rat
• Cited Reactivity: human, mouse, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CLTC fusion protein Ag25041
• Full Name: clathrin, heavy chain (Hc)
• Calculated molecular weight: 192 kDa
• Observed molecular weight: 180 kDa
• GenBank accession number: BC054489
• Gene symbol: CLTC
• Gene ID (NCBI): 1213
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (CLTC) (160-190 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The heavy chain provides the structural backbone of the clathrin lattice. (PMID: 9133677)

Publications

Species	Application	Title
human	WB	Autophagy; AP2M1 mediates autophagy-induced CLDN2 (claudin 2) degradation through endocytosis and interaction with LC3 and reduces intestinal epithelial tight junction permeability.; Authors - Ashwinkumar Subramenium Ganapathy
human	IF	Br J Pharmacol; A glycine-serine-alanine-rich peptide ameliorates Alzheimer-type neurodegeneration; Authors - Si-Yuan Yao
mouse	IF	Aging (Albany NY); The interaction protein of SORBS2 in myocardial tissue to find out the pathogenic mechanism of LVNC disease.; Authors - Chunyan Li
bovine	WB,IF	Vet Microbiol; Invasion of Mycoplasma bovis into bovine synovial cells utilizing the clathrin-dependent endocytosis pathway.; Authors - Koji Nishi ; KD Validated

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