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CLTC Polyclonal antibody

CLTC Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, pig, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 26523-1-AP

Synonyms

CHC, CHC17, Clathrin heavy chain 1, clathrin, heavy chain (Hc), CLH 17, CLH17, CLTC, CLTCL2, Hc, KIAA0034



Tested Applications

Positive WB detected inHeLa cells, HL-60 cells, pig brain tissue, Raji cells, mouse brain tissue, rat brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:250-1:1000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

26523-1-AP targets CLTC in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, pig, mouse, rat samples.

Tested Reactivity human, pig, mouse, rat
Cited Reactivityhuman, mouse, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLTC fusion protein Ag25041
Full Name clathrin, heavy chain (Hc)
Calculated molecular weight 192 kDa
Observed molecular weight 180 kDa
GenBank accession numberBC054489
Gene symbol CLTC
Gene ID (NCBI) 1213
RRIDAB_2880542
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (CLTC) (160-190 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The heavy chain provides the structural backbone of the clathrin lattice. (PMID: 9133677)

Protocols

Product Specific Protocols
WB protocol for CLTC antibody 26523-1-APDownload protocol
IHC protocol for CLTC antibody 26523-1-APDownload protocol
IF protocol for CLTC antibody 26523-1-APDownload protocol
IP protocol for CLTC antibody 26523-1-APDownload protocol
FC protocol for CLTC antibody 26523-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Emerg Microbes Infect

CD1d facilitates African swine fever virus entry into the host cells via clathrin-mediated endocytosis

Authors - Xin Chen
humanWB

Autophagy

AP2M1 mediates autophagy-induced CLDN2 (claudin 2) degradation through endocytosis and interaction with LC3 and reduces intestinal epithelial tight junction permeability.

Authors - Ashwinkumar Subramenium Ganapathy
humanIF

Br J Pharmacol

A glycine-serine-alanine-rich peptide ameliorates Alzheimer-type neurodegeneration

Authors - Si-Yuan Yao
mouseIF

Aging (Albany NY)

The interaction protein of SORBS2 in myocardial tissue to find out the pathogenic mechanism of LVNC disease.

Authors - Chunyan Li
bovineWB,IF

Vet Microbiol

Invasion of Mycoplasma bovis into bovine synovial cells utilizing the clathrin-dependent endocytosis pathway.

Authors - Koji Nishi
  • KD Validated
humanIF

FASEB J

A genome-wide transposon mutagenesis screening identifies LppB as a key factor associated with Mycoplasma bovis colonization and invasion into host cells

Authors - Shimei Lan