Home
Products
Primary Antibodies
CRB1 Polyclonal antibody, PBS Only (26598-1-PBS)

Filter: AllIHCIF-PIF/ICC

IHC staining of mouse eye using 26598-1-AP (same clone as 26598-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 26598-1-AP (CRB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26598-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 26598-1-AP (same clone as 26598-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26598-1-AP (CRB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26598-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 26598-1-AP (same clone as 26598-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26598-1-AP (CRB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26598-1-PBS in a different storage buffer formulation.
IHC staining of mouse eye using 26598-1-AP (same clone as 26598-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 26598-1-AP (CRB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26598-1-PBS in a different storage buffer formulation.
IHC staining of mouse eye using 26598-1-AP (same clone as 26598-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 26598-1-AP (CRB1 antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26598-1-PBS in a different storage buffer formulation.
IHC staining of mouse eye using 26598-1-AP (same clone as 26598-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 26598-1-AP (CRB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26598-1-PBS in a different storage buffer formulation.
IF Staining of mouse eye using 26598-1-AP (same clone as 26598-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using CRB1 antibody (26598-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26598-1-PBS in a different storage buffer formulation.
IF Staining of SH-SY5Y using 26598-1-AP (same clone as 26598-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CRB1 antibody (26598-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26598-1-PBS in a different storage buffer formulation.

CRB1 Polyclonal antibody, PBS Only

Name: CRB1 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 26598-1-PBS

CRB1 Polyclonal Antibody for IHC, IF/ICC, IF-P, Indirect ELISA

Cat No. 26598-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

Crumbs homolog 1, crumbs homolog 1 (Drosophila), LCA8, Protein crumbs homolog 1, RP12

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

26598-1-PBS targets CRB1 in IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag24512 Product name: Recombinant human CRB1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 30-188 aa of BC136271 Sequence: NNTRCLSNSCQNNSTCKDFSKDNDCSCSDTANNLDKDCDNMKDPCFSNPCQGSATCVNTPGERSFLCKCPPGYSGTICETTIGSCGKNSCQHGGICHQDPIYPVCICPAGYAGRFCEIDHDECASSPCQNGAVCQDGIDGYSCFCVPGYQGRHCDLEVD Predict reactive species
Full Name	crumbs homolog 1 (Drosophila)
Calculated Molecular Weight	1406 aa, 154 kDa
Observed Molecular Weight	150 kDa
GenBank Accession Number	BC136271
Gene Symbol	CRB1
Gene ID (NCBI)	23418
RRID	AB_2918104
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P82279
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

CRB1, also known as protein crumbs homolog 1, is homologous to Drosophila Crumbs, a protein that is essential for establishing and maintaining apico-basal polarity in epithelia derived from the ectoderm (PMID: 2344615). CRB1 protein is a single transmembrane protein with a large extracellular part, containing three laminin A G domains and 19 epidermal growth-factor-like domains (PMID: 15494026, 18407265, 11734541). The expression of CRB1 is restricted to the retina and brain (PMID: 10508521). Mutations in CRB1 gene cause severe retinal dystrophies, ranging from retinitis pigmentosa (RP) to Leber congenital amaurosis (LCA) (PMID: 11734541).