CoraLite®594-conjugated CRP Monoclonal antibody

CRP Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3F10A11

Cat no : CL594-66250

Synonyms

C reactive protein, CRP, PTX1

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Validation Data Gallery

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  IF Staining of human liver using CL594-66250

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CL594-66250 (CRP antibody) at dilution of 1:100.

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Tested Applications

• Positive IF detected in: human liver tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66250 targets CRP in IF applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CRP fusion protein Ag9883
• Full Name: C-reactive protein, pentraxin-related
• Calculated Molecular Weight: 224 aa, 25 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC020766
• Gene Symbol: CRP
• Gene ID (NCBI): 1401
• RRID: AB_2883544
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C-reactive protein (CRP) is an acute-phase serum protein synthesized by the liver in response to interleukin-6 (IL-6) during inflammation. The name of CRP derives from its ability to react with the C polysaccharide of Streptococcus pneumoniae. CRP is an annular, pentameric protein that belongs to the pentraxin family of proteins. CRP displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. It is used mainly as a marker of inflammation.