Tested Applications
| Positive WB detected in | Human heart, pig heart tissue, human heart tissue, Transfected HEK-293 cells, rabbit heart tissue |
| Positive IHC detected in | human heart tissue, human bladder tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60228-1-Ig targets Cytoglobin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4161 Product name: Recombinant human CYGB protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1-190 aa of BC029798 Sequence: MEKVPGEMEIERRERSEELSEAERKAVQAMWARLYASCEDVGVAILVRFFVNFPSAKQYFSQFKHMEDPLEMERSPQLRKHACRVMGALNTVVENLHDPDKVSSVLALVGKAHALKHKVEPVYFKILSGVILEVVAEEFASDFPPETQRAWAKLRGLIYSHVTAAYKEVGWVQQVPNATTPPATLPSSGP Predict reactive species |
| Full Name | cytoglobin |
| Calculated Molecular Weight | 190 aa, 21 kDa |
| Observed Molecular Weight | 27-29 kDa |
| GenBank Accession Number | BC029798 |
| Gene Symbol | Cytoglobin |
| Gene ID (NCBI) | 114757 |
| RRID | AB_11182383 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q8WWM9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Cytoglobin, also named as Histoglobin, HGb, STAP and CYGB, belongs to the globin family. Cytoglobin may have a protective function during conditions of oxidative stress. It may be involved in intracellular oxygen storage or transfer. It serves as a defensive mechanism against oxidative stress both in vitro and in vivo.(PMID:21224051). Cytoglobin is a ubiquitously expressed hexacoordinate hemoglobin that may facilitate diffusion of oxygen through tissues. The protein, with calculated MW of 21 kDa, runs as a 29 kDa one in canine retina, kidney, liver, lung, and heart (PMID: 16505036). It may be caused by posttranslational modification of the protein. Various immuno-staining patterns were reported including nuclear, cytoplasm and extracellular location.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Cytoglobin antibody 60228-1-Ig | Download protocol |
| IHC protocol for Cytoglobin antibody 60228-1-Ig | Download protocol |
| WB protocol for Cytoglobin antibody 60228-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Cytoglobin Monoclonal antibody (Cat# 60228-1-Ig) has 5 citations published in Cell Death & Disease, Neural Regen Res, Molecular Medicine Reports, Journal of Cellular and Molecular Medicine, and Molecular and Cellular Biochemistry. The associated research fields include head and neck squamous cell carcinoma, neurodegeneration and cognitive function, melanoma, ferroptosis, and colorectal cancer, with applications spanning Western blot, immunofluorescence, and immunoprecipitation in human and mouse models.
| Species | Application | Title |
|---|---|---|
Cell Death Dis ΔNp63α promotes Bortezomib resistance via the CYGB-ROS axis in head and neck squamous cell carcinoma. | ||
Neural Regen Res Treadmill exercise in combination with acousto-optic and olfactory stimulation improves cognitive function in APP/PS1 mice through the brain-derived neurotrophic factor- and Cygb-associated signaling pathways | ||
Mol Med Rep Cytoglobin attenuates melanoma malignancy but protects melanoma cells from ferroptosis | ||
Mol Cell Biochem Cytoglobin augments ferroptosis through autophagic degradation of ferritin in colorectal cancer cells | ||
J Cell Mol Med Cytoglobin promotes sensitivity to ferroptosis by regulating p53-YAP1 axis in colon cancer cells. |
