Cytoglobin Monoclonal antibody

Cytoglobin Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1


human, mouse, pig, rat







Cat no : 60228-1-Ig


CYGB, cytoglobin, HGB, Histoglobin, STAP

Tested Applications

Positive WB detected inHuman heart, pig heart tissue, human heart tissue, Transfected HEK-293 cells, rabbit heart tissue
Positive IHC detected inhuman liver tissue, human heart tissue, human bladder tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

60228-1-Ig targets Cytoglobin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, pig, rat samples.

Tested Reactivity human, mouse, pig, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cytoglobin fusion protein Ag4161
Full Name cytoglobin
Calculated molecular weight 190 aa, 21 kDa
Observed molecular weight 27-29 kDa
GenBank accession numberBC029798
Gene symbol CYGB
Gene ID (NCBI) 114757
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cytoglobin is a ubiquitously expressed hexacoordinate hemoglobin that may facilitate diffusion of oxygen through tissues. The protein, with calculated MW of 21 kDa, runs as a 29 kDa one in canine retina, kidney, liver, lung, and heart. It may be caused by posttranslational modification of the protein. Various immuno-staining patterns were reported including nuclear, cytoplasm and extracellular location.


Product Specific Protocols
WB protocol for Cytoglobin antibody 60228-1-IgDownload protocol
IHC protocol for Cytoglobin antibody 60228-1-IgDownload protocol
IF protocol for Cytoglobin antibody 60228-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Cell Mol Med

Cytoglobin promotes sensitivity to ferroptosis by regulating p53-YAP1 axis in colon cancer cells.

Authors - Shazhou Ye

Cell Death Dis

ΔNp63α promotes Bortezomib resistance via the CYGB-ROS axis in head and neck squamous cell carcinoma.

Authors - Peng Zhou