Cytoglobin Antibody

Cytoglobin Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

4C7E11

Cat no : 60228-1-Ig

Synonyms

CYGB, cytoglobin, HGB, Histoglobin



Tested Applications

Positive WB detected inHuman heart, tissue
Positive IHC detected inhuman liver tissue, human bladder tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The 60228-1-Ig immunogen is Fusion Protein Cytoglobin protein expressed in E. coli.

Tested Reactivity human, mouse, pig, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cytoglobin fusion protein Ag4161
Full Name cytoglobin
Calculated molecular weight 190aa,21 kDa
Observed molecular weight 27-29 kDa
GenBank accession number BC029798
Gene symbol CYGB
Gene ID (NCBI) 114757
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cytoglobin is a ubiquitously expressed hexacoordinate hemoglobin that may facilitate diffusion of oxygen through tissues. The protein, with calculated MW of 21 kd, runs as a 29kd one in canine retina, kidney, liver, lung, and heart. It may be caused by posttranslational modification of the protein. Various immuno-staining patterns were reported including nuclear, cytoplasm and extracellular location.

Protocols

Product Specific Protocols
WB protocol for Cytoglobin antibody 60228-1-IgDownload protocol
IHC protocol for Cytoglobin antibody 60228-1-IgDownload protocol
IF protocol for Cytoglobin antibody 60228-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols