CoraLite® Plus 488-conjugated Cytoglobin Monoclonal antibody

Cytoglobin Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4C7E11

Cat no : CL488-60228

Synonyms

CYGB, cytoglobin, HGB, Histoglobin, STAP



Tested Applications

Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

CL488-60228 targets Cytoglobin in IF applications and shows reactivity with human, mouse, pig, rat samples.

Tested Reactivity human, mouse, pig, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cytoglobin fusion protein Ag4161
Full Name cytoglobin
Calculated molecular weight 190 aa, 21 kDa
Observed molecular weight 27-29 kDa
GenBank accession numberBC029798
Gene symbol CYGB
Gene ID (NCBI) 114757
RRIDAB_2883122
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cytoglobin is a ubiquitously expressed hexacoordinate hemoglobin that may facilitate diffusion of oxygen through tissues. The protein, with calculated MW of 21 kDa, runs as a 29 kDa one in canine retina, kidney, liver, lung, and heart. It may be caused by posttranslational modification of the protein. Various immuno-staining patterns were reported including nuclear, cytoplasm and extracellular location.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Cytoglobin antibody CL488-60228Download protocol
Standard Protocols
Click here to view our Standard Protocols