Tested Applications
| Positive WB detected in | Caco-2 cells, HepG2 cells, HT-29 cells, rat liver tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human liver cancer tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67045-1-Ig targets CYP27A1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7746 Product name: Recombinant human CYP27A1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 183-531 aa of BC051851 Sequence: DDFMTRLDQLRAESASGNQVSDMAQLFYYFALEAICYILFEKRIGCLQRSIPEDTVTFVRSIGLMFQNSLYATFLPKWTRPVLPFWKRYLDGWNAIFSFGKKLIDEKLEDMEAQLQAAGPDGIQVSGYLHFLLASGQLSPREAMGSLPELLMAGVDTTSNTLTWALYHLSKDPEIQEALHEEVVGVVPAGQVPQHKDFAHMPLLKAVLKETLRLYPVVPTNSRIIEKEIEVDGFLFPKNTQFVFCHYVVSRDPTAFSEPESFQPHRWLRNSQPATPRIQHPFGSVPFGYGVRACLGRRIAELEMQLLLARLIQKYKVVLAPETGELKSVARIVLVPNKKVGLQFLQRQC Predict reactive species |
| Full Name | cytochrome P450, family 27, subfamily A, polypeptide 1 |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 54-60 kDa |
| GenBank Accession Number | BC051851 |
| Gene Symbol | CYP27A1 |
| Gene ID (NCBI) | 1593 |
| RRID | AB_2882359 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q02318 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CYP27A1 antibody 67045-1-Ig | Download protocol |
| IHC protocol for CYP27A1 antibody 67045-1-Ig | Download protocol |
| WB protocol for CYP27A1 antibody 67045-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
CYP27A1 Monoclonal antibody (3G3F7), catalog number 67045-1-Ig, has 10 citations published in journals including Nature Microbiology, Advanced Science, Materials Today Bio, Journal of Animal Science and Biotechnology, Frontiers in Immunology, Journal of Ethnopharmacology, Journal of Bone and Mineral Research, Nutrients, and Immunology. Research fields span liver disease, lipid metabolism, hepatocellular carcinoma, obesity, infectious disease, cancer immunotherapy, vitamin D-dependent rickets, and asthma.
| Species | Application | Title |
|---|---|---|
Nat Microbiol Akkermansia muciniphila protects mice against an emerging tick-borne viral pathogen | ||
Adv Sci (Weinh) Geniposidic Acid Targeting FXR "S332 and H447" Mediated Conformational Change to Upregulate CYPs and miR-19a-3p to Ameliorate Drug-Induced Liver Injury | ||
Mater Today Bio Integrating gene demethylation and immune modulation: PD-1 nanovesicles as a dual-action therapy for NSCLC | ||
J Anim Sci Biotechnol Taurolithocholic acid alleviates hepatic glycolipid dysregulation in low-birth-weight piglets by activating HNF4α and FXR. | ||
Front Immunol Construction of a lipid metabolism-related risk model for hepatocellular carcinoma by single cell and machine learning analysis | ||
J Ethnopharmacol Penthorum chinense Pursh. extract attenuates non-alcholic fatty liver disease by regulating gut microbiota and bile acid metabolism in mice. |
