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CoraLite®594-conjugated CYP27A1 Monoclonal antibody

CYP27A1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3G3F7

Cat no : CL594-67045

Synonyms

CP27, CTX, CYP27, CYP27A1, Cytochrome P 450C27/25, Cytochrome P450 27, Sterol 27 hydroxylase, Vitamin D(3) 25 hydroxylase

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using CoraLite®594-conjugated CYP27A1 Monoclonal antibod (CL594-67045)

    IF Staining of human liver cancer using CL594-67045

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 CYP27A1 antibody (CL594-67045, Clone: 3G3F7 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using CoraLite®594-conjugated CYP27A1 Monoclonal antibod (CL594-67045)

    IF Staining of human liver cancer using CL594-67045

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 CYP27A1 antibody (CL594-67045, Clone: 3G3F7 ) at dilution of 1:200.

Tested Applications

Positive IF detected inhuman liver cancer tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67045 targets CYP27A1 in IF applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CYP27A1 fusion protein Ag7746
Full Name cytochrome P450, family 27, subfamily A, polypeptide 1
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 54-60 kDa
GenBank Accession NumberBC051851
Gene Symbol CYP27A1
Gene ID (NCBI) 1593
RRIDAB_2920079
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for CL594 CYP27A1 antibody CL594-67045Download protocol
Standard Protocols
Click here to view our Standard Protocols