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Cyclin B1 Polyclonal antibody, PBS Only (55004-1-PBS)

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 55004-1-AP (same clone as 55004-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 55004-1-AP (same clone as 55004-1-PBS)
WB result of Cyclin B1 antibody (55004-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin B1 transfected HeLa cells. This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 55004-1-AP (same clone as 55004-1-PBS)
WB result of Cyclin B1 antibody (55004-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin B1 transfected HeLa cells. This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.
WB analysis using 55004-1-AP (same clone as 55004-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 55004-1-AP (same clone as 55004-1-PBS)
Non-treated HeLa and thymidine or nocodazole treated HeLa cells were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.
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Non-treated HeLa and thymidine or nocodazole treated HeLa cells were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.
WB analysis using 55004-1-AP (same clone as 55004-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.
WB analysis of NIH/3T3 using 55004-1-AP (same clone as 55004-1-PBS)
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 55004-1-AP (same clone as 55004-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 55004-1-AP (same clone as 55004-1-PBS)
IP result of anti-Cyclin B1 (IP:55004-1-AP, 4ug; Detection:55004-1-AP 1:2000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.
IHC staining of human skin cancer using 55004-1-AP (same clone as 55004-1-PBS)
Immunohistochemical analysis of paraffin-embedded skin cancer slide using 55004-1-AP (Cyclin B1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.
IHC staining of human skin cancer using 55004-1-AP (same clone as 55004-1-PBS)
Immunohistochemical analysis of paraffin-embedded skin cancer slide using 55004-1-AP (Cyclin B1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 55004-1-AP (same clone as 55004-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cyclin B1 antibody (55004-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.
IF Staining of U2OS using 55004-1-AP (same clone as 55004-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using Cyclin B1 antibody (55004-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.
FC experiment of HeLa using 55004-1-AP (same clone as 55004-1-PBS)
1x10^6 HeLa cells were intracellularly stained with 0.4 ug Cyclin B1 Polyclonal antibody (55004-1-AP)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

Cyclin B1 Polyclonal antibody, PBS Only

Name: Cyclin B1 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 55004-1-PBS

Cyclin B1 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, Indirect ELISA

Cat No. 55004-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

CCNB1, G2/mitotic-specific cyclin-B1, G2/mitotic specific cyclin B1, Cyclin B, CCNB

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

55004-1-PBS targets Cyclin B1 in WB, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	cyclin B1
Calculated Molecular Weight	48 kDa
Observed Molecular Weight	55-60 kDa
GenBank Accession Number	NM_031966
Gene Symbol	Cyclin B1
Gene ID (NCBI)	891
RRID	AB_10859790
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P14635
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.