Cyclophilin B Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Cyclophilin B Monoclonal Antibody for WB, IF, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

8H4F1

Cat no : 66047-1-PBS

Synonyms

Cyclophilin B, CYP S1, CYPB, PPIase B, PPIB, Rotamase B, S cyclophilin, SCYLP

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Validation Data Gallery

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  WB analysis of HeLa using 66047-1-Ig (same clone as 66047-1-PBS)

  WB result of Cyclophilin B antibody (66047-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclophilin B transfected HeLa cells. This data was developed using the same antibody clone with 66047-1-PBS in a different storage buffer formulation.

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  WB analysis using 66047-1-Ig (same clone as 66047-1-PBS)

  HeLa/Jurkat/ROS1728/PC-12/C6 cells were subjected to SDS PAGE followed by western blot with 66047-1-Ig (Cyclophilin B antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66047-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 66047-1-Ig (same clone as 66047-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66047-1-Ig (Cyclophilin B antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66047-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66047-1-Ig (same clone as 66047-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66047-1-Ig (Cyclophilin B antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66047-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66047-1-Ig (same clone as 66047-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66047-1-Ig (Cyclophilin B antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66047-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66047-1-Ig (same clone as 66047-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66047-1-Ig (Cyclophilin B antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66047-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66047-1-Ig (same clone as 66047-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66047-1-Ig (Cyclophilin B antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66047-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 66047-1-Ig (same clone as 66047-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66047-1-Ig (Cyclophilin B antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66047-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 66047-1-Ig (same clone as 66047-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66047-1-Ig (Cyclophilin B antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66047-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 66047-1-Ig (same clone as 66047-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 66047-1-Ig(Cyclophilin B antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66047-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 66047-1-Ig (same clone as 66047-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 66047-1-Ig(Cyclophilin B antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66047-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66047-1-Ig (same clone as 66047-1-PBS)

  Immunofluorescent analysis of (4% PFA ) fixed HeLa cells using 66047-1-Ig(Cyclophilin B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66047-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66047-1-Ig (same clone as 66047-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cyclophilin B antibody (66047-1-Ig, Clone: 8H4F1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66047-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 66047-1-Ig (same clone as 66047-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Cyclophilin B (66047-1-Ig, Clone:8H4F1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66047-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66047-1-PBS targets Cyclophilin B in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Cyclophilin B fusion protein Ag17957
• Full Name: peptidylprolyl isomerase B (cyclophilin B)
• Calculated Molecular Weight: 216 aa, 24 kDa
• Observed Molecular Weight: 19-24 kDa
• GenBank Accession Number: BC032138
• Gene Symbol: PPIB
• Gene ID (NCBI): 5479
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PPIB, also named as CYPB, SCYLP, PPIase B and CYP-S1, belongs to the cyclophilin-type PPIase family and PPIase B subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIB is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes. The full-length of PPIB has 216aa (24 kDa), and a 33aa-signal peptide could be removed in the mature form (about 19 kDa).