Cyclophilin B Monoclonal antibody

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  WB analysis using 66047-1-Ig

  HeLa/Jurkat/ROS1728/PC-12/C6 cells were subjected to SDS PAGE followed by western blot with 66047-1-Ig (Cyclophilin B antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66047-1-Ig

  WB result of Cyclophilin B antibody (66047-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclophilin B transfected HeLa cells.

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  WB analysis of HeLa using 66047-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66047-1-Ig (Cyclophilin B antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 66047-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66047-1-Ig (Cyclophilin B antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66047-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66047-1-Ig (Cyclophilin B antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 66047-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66047-1-Ig (Cyclophilin B antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 66047-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66047-1-Ig (Cyclophilin B antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 66047-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66047-1-Ig (Cyclophilin B antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 66047-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66047-1-Ig (Cyclophilin B antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 66047-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 66047-1-Ig(Cyclophilin B antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas cancer using 66047-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 66047-1-Ig(Cyclophilin B antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 66047-1-Ig

  Immunofluorescent analysis of (4% PFA ) fixed HeLa cells using 66047-1-Ig(Cyclophilin B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HeLa using 66047-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cyclophilin B antibody (66047-1-Ig, Clone: 8H4F1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HeLa using 66047-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Cyclophilin B (66047-1-Ig, Clone:8H4F1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

• Featured Product
• KD/KO Validated

Cyclophilin B Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 66047-1-Ig

Clone No.8H4F1

Publications(1)

Reviews (1)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

PPIB, 8H4F1, CYP S1, CYPB, CYP-S1

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, HEK-293 cells, Jurkat cells, RAW 264.7 cells, ROS1728 cells, PC-12 cells, C6 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

66047-1-Ig targets Cyclophilin B in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17957

  Product name: Recombinant human PPIB protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-216 aa of BC032138

  Sequence: MLRLSERNMKVLLAAALIAGSVFFLLLPGPSAADEKKKGPKVTVKVYFDLRIGDEDVGRVIFGLFGKTVPKTVDNFVALATGEKGFGYKNSKFHRVIKDFMIQGGDFTRGDGTGGKSIYGERFPDENFKLKHYGPGWVSMANAGKDTNGSQFFITTVKTAWLDGKHVVFGKVLEGMEVVRKVESTKTDSRDKPLKDVIIADCGKIEVEKPFAIAKE

• Full Name: peptidylprolyl isomerase B (cyclophilin B)
• Calculated Molecular Weight: 216 aa, 24 kDa
• Observed Molecular Weight: 19-24 kDa
• GenBank Accession Number: BC032138
• Gene Symbol: Cyclophilin B
• Gene ID (NCBI): 5479
• RRID: AB_11042327
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P23284
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPIB, also named as CYPB, SCYLP, PPIase B and CYP-S1, belongs to the cyclophilin-type PPIase family and PPIase B subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIB is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes. The full-length of PPIB has 216aa (24 kDa), and a 33aa-signal peptide could be removed in the mature form (about 19 kDa).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Mol Ther Nucleic Acids; Design of bifunctional RNA-binding compounds that can modulate siRNA hydrophobicity for deep penetration and gene silencing in the retina.; Authors - Qi Mao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Leon (Verified Customer) (07-06-2022)

Seems like a good antibody that detects cyclophilin B well and gave clear bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: SH-SY5Y

Cyclophilin B Antibody Western Blot validation (1:5000 dilution) in SH-SY5Y (Cat no:66047-1-Ig)