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- KD/KO Validated
Cyclophilin B Monoclonal antibody
Cyclophilin B Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2a
WB, IHC, IF, FC, ELISA
Cat no : 66047-1-Ig
Validation Data Gallery
|Positive WB detected in
|HeLa cells, MCF-7 cells, HEK-293 cells, Jurkat cells, RAW 264.7 cells, ROS1728 cells, PC-12 cells, C6 cells
|Positive IHC detected in
|human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Positive FC detected in
|Western Blot (WB)
|WB : 1:1000-1:8000
|IHC : 1:250-1:1000
|IF : 1:50-1:500
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
66047-1-Ig targets Cyclophilin B in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
|Host / Isotype
|Mouse / IgG2a
|Cyclophilin B fusion protein Ag17957
|peptidylprolyl isomerase B (cyclophilin B)
|Calculated molecular weight
|216 aa, 24 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein A purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
PPIB, also named as CYPB, SCYLP, PPIase B and CYP-S1, belongs to the cyclophilin-type PPIase family and PPIase B subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIB is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes. The full-length of PPIB has 216aa (24 kDa), and a 33aa-signal peptide could be removed in the mature form (about 19 kDa).
|Product Specific Protocols
|WB protocol for Cyclophilin B antibody 66047-1-Ig
|IHC protocol for Cyclophilin B antibody 66047-1-Ig
|IF protocol for Cyclophilin B antibody 66047-1-Ig
|FC protocol for Cyclophilin B antibody 66047-1-Ig
|Click here to view our Standard Protocols
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Leon (Verified Customer) (07-06-2022)
Seems like a good antibody that detects cyclophilin B well and gave clear bands.