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Cyclophilin B Monoclonal antibody
Cyclophilin B Monoclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Mouse / IgG2a
WB, IHC, IF,ELISA
Cat no : 66047-1-Ig
Validation Data Gallery
|Positive WB detected in||HeLa cells, MCF-7 cells, HEK-293 cells, Jurkat cells, RAW 264.7 cells|
|Positive IHC detected in||human pancreas cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunohistochemistry (IHC)||IHC : 1:250-1:1000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 66047-1-Ig is Cyclophilin B Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Cyclophilin B fusion protein Ag17957|
|Full Name||peptidylprolyl isomerase B (cyclophilin B)|
|Calculated molecular weight||216 aa, 24 kDa|
|Observed molecular weight||19-24 kDa|
|GenBank accession number||BC032138|
|Gene ID (NCBI)||5479|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PPIB, also named as CYPB, SCYLP, PPIase B and CYP-S1, belongs to the cyclophilin-type PPIase family and PPIase B subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIB is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes. The full-length of PPIB has 216aa (24 kDa), and a 33aa-signal peptide could be removed in the mature form (about 19 kDa).
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Leon (Verified Customer) (07-06-2022)
Seems like a good antibody that detects cyclophilin B well and gave clear bands.