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  • KD/KO Validated

Cyclophilin B Monoclonal antibody

Cyclophilin B Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a


human, mouse







Cat no : 66047-1-Ig


Cyclophilin B, CYP S1, CYPB, PPIase B, PPIB, Rotamase B, S cyclophilin, SCYLP

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, HEK-293 cells, Jurkat cells, RAW 264.7 cells, ROS1728 cells, PC-12 cells, C6 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

66047-1-Ig targets Cyclophilin B in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Cyclophilin B fusion protein Ag17957
Full Name peptidylprolyl isomerase B (cyclophilin B)
Calculated molecular weight 216 aa, 24 kDa
Observed molecular weight 19-24 kDa
GenBank accession numberBC032138
Gene symbol PPIB
Gene ID (NCBI) 5479
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPIB, also named as CYPB, SCYLP, PPIase B and CYP-S1, belongs to the cyclophilin-type PPIase family and PPIase B subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIB is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes. The full-length of PPIB has 216aa (24 kDa), and a 33aa-signal peptide could be removed in the mature form (about 19 kDa).


Product Specific Protocols
WB protocol for Cyclophilin B antibody 66047-1-IgDownload protocol
IHC protocol for Cyclophilin B antibody 66047-1-IgDownload protocol
IF protocol for Cyclophilin B antibody 66047-1-IgDownload protocol
FC protocol for Cyclophilin B antibody 66047-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Leon (Verified Customer) (07-06-2022)

Seems like a good antibody that detects cyclophilin B well and gave clear bands.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: SH-SY5Y
Cyclophilin B Antibody Western Blot validation (1:5000 dilution) in SH-SY5Y (Cat no:66047-1-Ig)