DNASE1L3 Monoclonal antibody

DNASE1L3 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1A4D4

Reviews (1)

Cat no : 67041-1-Ig

Synonyms

Deoxyribonuclease gamma, deoxyribonuclease I like 3, DHP2, DNAS1L3, DNase gamma, DNase I homolog protein DHP2, DNase I like 3, DNASE1L3, Liver and spleen DNase, LS DNase, LSD

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Validation Data Gallery

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  WB analysis using 67041-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67041-1-Ig (DNASE1L3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67041-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67041-1-Ig (DNASE1L3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Daudi using 67041-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 67041-1-Ig (DNASE1L3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 67041-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67041-1-Ig (DNASE1L3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 67041-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67041-1-Ig (DNASE1L3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 67041-1-Ig

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67041-1-Ig (DNASE1L3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 67041-1-Ig

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67041-1-Ig (DNASE1L3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, U2OS cells, Daudi cells, A431 cells, LnCaP cells, Jurkat cells, K-562 cells, HepG2 cells, Raji cells
• Positive IHC detected in: mouse liver tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

67041-1-Ig targets DNASE1L3 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: DNASE1L3 fusion protein Ag28187
• Full Name: deoxyribonuclease I-like 3
• Calculated Molecular Weight: 305 aa, 36 kDa
• Observed Molecular Weight: 33-38 kDa
• GenBank Accession Number: BC015831
• Gene Symbol: DNASE1L3
• Gene ID (NCBI): 1776
• RRID: AB_2882355
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Deoxyribonuclease 1-like 3 (DNASE1L3, DNase gamma, DNase Y, LS-DNase) is member of a DNASE1 protein family that is identified by similar biochemical properties such as Ca2+/Mg2+- dependency and an optimal pH of about 7.0 as well as by a high similarity in their nucleic acid and amino acid sequences (PMID:157967140). It is an endonuclease and cleaves double-stranded DNA (and single-stranded DNA) producing 3′-OH/5′-P ends, which is Ca2+/Mg2+-dependent and inhibited by Zn2+, but not by monomeric actin (PMID:9665719). In addition, it translocates from the endoplasmic reticulum to the nucleus during apoptosis (PMID: 23229555). DNASE1L3 can be located either in the cytoplasm or in the nucleus.

Publications

Species	Application	Title
human	WB	Aging (Albany NY); Deoxyribonuclease 1-like 3 may be a potential prognostic biomarker associated with immune infiltration in colon cancer.; Authors - Jing Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tsz Tung (Verified Customer) (04-28-2022)

Work for mouse tumor-bearing liver FFPE-processed tissue (Red: DNase1L3)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse liver FFPE

DNASE1L3 Antibody Immunofluorescence validation (1:100 dilution) in Mouse liver FFPE (Cat no:67041-1-Ig)