DRD2 Polyclonal antibody, PBS Only

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• DRD2 Polyclonal antibody, PBS Only (55084-1-PBS)

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  WB analysis using 55084-1-AP (same clone as 55084-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55084-1-AP (DRD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55084-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 55084-1-AP (same clone as 55084-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55084-1-AP (DRD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55084-1-PBS in a different storage buffer formulation.

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  IP experiment of SH-SY5Y using 55084-1-AP (same clone as 55084-1-PBS)

  IP result of anti-DRD2 (IP:55084-1-AP, 4ug; Detection:55084-1-AP 1:300) with SH-SY5Y cells lysate 3000ug. This data was developed using the same antibody clone with 55084-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 55084-1-AP (same clone as 55084-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DRD2 antibody (55084-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55084-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 55084-1-AP (same clone as 55084-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DRD2 antibody (55084-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55084-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 55084-1-AP (same clone as 55084-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using DRD2 antibody (55084-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55084-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 55084-1-AP (same clone as 55084-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using 55084-1-AP (DRD2 antibody) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55084-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

DRD2 Polyclonal Antibody for WB, IF-P, IF-Fro, IP, Indirect ELISA

Cat No. 55084-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, IF-Fro, IP, Indirect ELISA

Synonyms

D(2) dopamine receptor, D2 dopamine receptor, D2DR, D2R, Dopamine D2 receptor

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Product Information

55084-1-PBS targets DRD2 in WB, IF-P, IF-Fro, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: dopamine receptor D2
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: NM_016574
• Gene Symbol: DRD2
• Gene ID (NCBI): 1813
• RRID: AB_10859941
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P14416
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

DRD2, also named D2DR and D2R, belongs to the G-protein coupled receptor 1 family. It is one of the five types (D1 to D5) of receptors for dopamine. The activity of DRD2 is mediated by G proteins which inhibit adenylyl cyclase. Defects in DRD2 are associated with dystonia type 11 (DYT11). The antibody is specific to DRD2.