CoraLite® Plus 488-conjugated EEF1A1 Recombinant antibody
EEF1A1 Recombinant Antibody for FC (Intra), IF
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse, Rat
Applications
IF, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2B16
Cat no : CL488-81377
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | HepG2 cells |
Positive FC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-81377 targets EEF1A1 in IF, FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.
Tested Reactivity | Human, Mouse, Rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | EEF1A1 fusion protein Ag1938 |
Full Name | eukaryotic translation elongation factor 1 alpha 1 |
Calculated Molecular Weight | 462 aa, 50 kDa |
Observed Molecular Weight | 50 kDa |
GenBank Accession Number | BC014224 |
Gene Symbol | EEF1A1 |
Gene ID (NCBI) | 1915 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
EEF1A1, also named as EEF1A, EF1A and LENG7, belongs to the GTP-binding elongation factor family. This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. It is a typical housekeeping gene product required for the maintenance of cell proliferation and/or survival. In addition, EEF1A1 protects the aminoester bond against hydrolysis until a correct match between the codon on mRNA and the anti-codon on tRNA can be achieved.
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 EEF1A1 antibody CL488-81377 | Download protocol |
FC protocol for CL Plus 488 EEF1A1 antibody CL488-81377 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |