CoraLite® Plus 488-conjugated FBL Monoclonal antibody

FBL Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3A9E8

Cat no : CL488-66985

Synonyms

FBL, FIB, FIB1, fibrillarin, FLRN, RNU3IP1

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-66985

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 FBL antibody (CL488-66985, Clone: 3A9E8 ) at dilution of 1:100.

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  IF Staining of HeLa using CL488-66985

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 FBL antibody (CL488-66985, Clone: 3A9E8 ) at dilution of 1:200, Alpha Tubulin antibody (11224-1-AP, red).

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  FC experiment of HepG2 using CL488-66985

  1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human FBL (CL488-66985, Clone:3A9E8) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IF detected in: HeLa cells, HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66985 targets FBL in IF, FC (Intra) applications and shows reactivity with Human, rat, mouse samples.

• Tested Reactivity: Human, rat, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FBL fusion protein Ag8841
• Full Name: fibrillarin
• Calculated Molecular Weight: 321 aa, 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC019260
• Gene Symbol: FBL
• Gene ID (NCBI): 2091
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fibrillarin (Fbl) is an essential nucleolar protein that plays an essential role in ribosome biogenesis and more particularly in the methylation of ribosomal RNAs and rDNA histones. FBL is highly expressed in various cancers and correlates with poor survival outcomes in cancer patients,such as hepatocellular carcinoma(PMID: 33376525). Knockdown of FBL sensitizes tumor cells and xenografts to DNA crosslinking agents, and leads to homologous recombination-mediated DNA repair defects(PMID: 37489617).