|Positive WB detected in||MCF-7 cells, T-47D cells, HEK-293 cells, human testis tissue, HeLa cells, MDA-MB-453s cells, human brain tissue, Jurkat cells|
|Positive IP detected in||MCF-7 cells|
|Positive IHC detected in||human testis tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10060-1-AP targets FKBPL in WB, IP, IHC, IF, ChIP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||FKBPL fusion protein Ag0112|
|Full Name||FK506 binding protein like|
|Calculated molecular weight||349 aa, 38 kDa|
|Observed molecular weight||42-48 kDa, 38-40 kDa|
|GenBank accession number||BC004168|
|Gene ID (NCBI)||63943|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
FKBPL, also named as DIR1, NG7 and WISp39, has similarity to the immunophilin protein family, which plays a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBPL levels may be a prognostic indicator and determinant of response to endocrine therapy(PMID:20103631, 15664193) .It can be detected the band between 38 Kda and 48 Kda by western blot.
FKBPL and its peptide derivatives inhibit endocrine therapy resistant cancer stem cells and breast cancer metastasis by downregulating DLL4 and Notch4.
Reprod Biol Endocrinol
Alterations in the steroid hormone receptor co-chaperone FKBPL are associated with male infertility: a case-control study.
Biol Sex Differ
Characterisation of cardiac health in the reduced uterine perfusion pressure model and a 3D cardiac spheroid model, of preeclampsia.
The anti-migratory effects of FKBPL and its peptide derivative, AD-01: regulation of CD44 and the cytoskeletal pathway.
J Biol Chem
A novel FK506-like binding protein interacts with the glucocorticoid receptor and regulates steroid receptor signaling.