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  • KD/KO Validated

FKBPL Polyclonal antibody

FKBPL Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, IF, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 10060-1-AP

Synonyms

DIR1, FK506 binding protein like, FKBPL, NG7, WISP39



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, human brain tissue, human testis tissue, Jurkat cells, MCF-7 cells, T-47D cells, MDA-MB-453s cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

10060-1-AP targets FKBPL in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FKBPL fusion protein Ag0112
Full Name FK506 binding protein like
Calculated molecular weight 349 aa, 38 kDa
Observed molecular weight 42-48 kDa
GenBank accession numberBC004168
Gene symbol FKBPL
Gene ID (NCBI) 63943
RRIDAB_2262677
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FKBPL, also named as DIR1, NG7 and WISp39, has similarity to the immunophilin protein family, which plays a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBPL levels may be a prognostic indicator and determinant of response to endocrine therapy(PMID:20103631, 15664193). It can be detected the band between 38 kDa and 48 kDa by western blot.

Protocols

Product Specific Protocols
WB protocol for FKBPL antibody 10060-1-APDownload protocol
IHC protocol for FKBPL antibody 10060-1-APDownload protocol
IP protocol for FKBPL antibody 10060-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF

J Adv Res

Hsa_circ_0001402 alleviates vascular neointimal hyperplasia through a miR-183-5p-dependent regulation of vascular smooth muscle cell proliferation, migration, and autophagy

Authors - Jia-Jie Lin
humanWB

Cancer Res

FKBPL regulates estrogen receptor signaling and determines response to endocrine therapy.

Authors - McKeen Hayley D HD
humanIF

Cell Mol Life Sci

A placenta-on-a-chip model to determine the regulation of FKBPL and galectin-3 in preeclampsia

Authors - Sahar Masoumeh Ghorbanpour
humanWB

J Control Release

Delivery of RALA/siFKBPL nanoparticles via electrospun bilayer nanofibres: An innovative angiogenic therapy for wound repair.

Authors - Eoghan J Mulholland
  • KD Validated
humanWB,IHC

Clin Cancer Res

FKBPL and peptide derivatives: novel biological agents that inhibit angiogenesis by a CD44-dependent mechanism.

Authors - Valentine Andrea A
humanWB, ChIP

Oncogene

Identification of RBCK1 as a novel regulator of FKBPL: implications for tumor growth and response to tamoxifen.

Authors - C Donley