CoraLite®594-conjugated Flightless I Monoclonal antibody

Flightless I Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2F9C8

Cat no : CL594-67039

Synonyms

FLI, Fli1, FLII, FLIL, Protein flightless 1 homolog

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Validation Data Gallery

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  IF Staining of HeLa using CL594-67039

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594-conjugated Flightless I antibody (CL594-67039, Clone: 2F9C8 ) at dilution of 1:100.

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Tested Applications

• Positive IF detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67039 targets Flightless I in IF applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Flightless I fusion protein Ag26865
• Full Name: flightless I homolog (Drosophila)
• Calculated Molecular Weight: 1269 aa, 145 kDa
• Observed Molecular Weight: 145-150 kDa
• GenBank Accession Number: BC025300
• Gene Symbol: FLII
• Gene ID (NCBI): 2314
• RRID: AB_2920077
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Flightless I (FliI) is the most evolutionarily conserved member of the gelsolin superfamily of proteins which are key regulators of actin filament assembly and turnover. FliI comprises an N-terminal leucine-rich repeat (LRR) domain which is not present in other gelsolin family members, and the LRR domain may enable interactions between FliI and other molecules involved in signal transduction, thereby spatially integrating signaling and actin remodeling functions. This protein was originally found in Drosophila and participates in the embryonic development, while mammalian FliI protein was involved in the regulation of wound repair,skin barrier development. Studies recently demonstrated that FliI protein associated with colorectal cancer, hepatocellular and prostate cancer (PMID:30091651; 28498392).