CoraLite® Plus 488-conjugated FRS2 Monoclonal antibody
FRS2 Monoclonal Antibody for FC (Intra), IF
Host / Isotype
Mouse / IgG3
Reactivity
human
Applications
IF, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
4E10H12
Cat no : CL488-66263
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | MCF-7 cells |
Positive FC detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
CL488-66263 targets FRS2 in IF, FC (Intra) applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG3 |
Class | Monoclonal |
Type | Antibody |
Immunogen | FRS2 fusion protein Ag19299 |
Full Name | fibroblast growth factor receptor substrate 2 |
Calculated molecular weight | 60 kDa |
Observed molecular weight | 68 kDa |
GenBank accession number | BC021562 |
Gene symbol | FRS2 |
Gene ID (NCBI) | 10818 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Fibroblast growth factor (FGF) receptor substrate 2 (FRS2) has an alternative name as SNT-1, it is an adapter protein that links activated FGR and NGF receptors to downstream signaling pathways. FGF receptor substrates (FRS2 and FRS3) are key adaptor proteins that mediate FGF-FGFR signalling in benign as well as malignant tissue. FRS2 is a 508 amino-acid protein,which is phosphorylated on tyrosine residues. The molecular weight of non-phosphorylated FRS2 is 57-68 kDa, but phosphorylated FRS2 is 80-90 kDa. Phosphorylation of FRS2 is associated with activation of a number of MAP kinases. Allele-specific regulation of FGFR2 mRNA expression with a mildly increased breast cancer risk has been reported.
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 FRS2 antibody CL488-66263 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |