GIGYF2 Polyclonal antibody, PBS Only

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• GIGYF2 Polyclonal antibody, PBS Only (24790-1-PBS)

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  WB analysis using 24790-1-AP (same clone as 24790-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24790-1-AP (GIGYF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24790-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 24790-1-AP (same clone as 24790-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 24790-1-AP (GIGYF2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24790-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 24790-1-AP (same clone as 24790-1-PBS)

  IP result of anti-GIGYF2 (IP:24790-1-AP, 4ug; Detection:24790-1-AP 1:2000) with Jurkat cells lysate 2000ug. This data was developed using the same antibody clone with 24790-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 24790-1-AP (same clone as 24790-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24790-1-AP (GIGYF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24790-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 24790-1-AP (same clone as 24790-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24790-1-AP (GIGYF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24790-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 24790-1-AP (same clone as 24790-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GIGYF2 antibody (24790-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 24790-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

GIGYF2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 24790-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

GRB10-interacting GYF protein 2, GYF2, KIAA0642, PERQ amino acid-rich with GYF domain-containing protein 2, PERQ2

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Product Information

24790-1-PBS targets GIGYF2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20329

  Product name: Recombinant human GIGYF2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 777-1104 aa of BC146775

  Sequence: KRQEELRRQQEEILRRQQEEERKRREEEELARRKQEEALRRQREQEIALRRQREEEERQQQEEALRRLEERRREEEERRKQEELLRKQEEEAAKWAREEEEAQRRLEENRLRMEEEAARLRHEEEERKRKELEVQRQKELMRQRQQQQEALRRLQQQQQQQQLAQMKLPSSSTWGQQSNTTACQSQATLSLAEIQKLEEERERQLREEQRRQQRELMKALQQQQQQQQQKLSGWGNVSKPSGTTKSLLEIQQEEARQMQKQQQQQQQHQQPNRARNNTHSNLHTSIGNSVWGSINTGPPNQWASDLVSSIWSNADTKNSNMGFWDDAV

• Full Name: GRB10 interacting GYF protein 2
• Calculated Molecular Weight: 1320 aa, 152 kDa
• Observed Molecular Weight: 150-170 kDa
• GenBank Accession Number: BC146775
• Gene Symbol: GIGYF2
• Gene ID (NCBI): 26058
• RRID: AB_2879727
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6Y7W6
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

GIGYF2 (GRB10 interacting GYF protein 2) was initially identified through its interaction with GRB10, an adapter protein that binds activated INS-like growth factor (IGF-I) and INS receptors (PMID: 12771153; 19744960). It may act cooperatively with GRB10 to regulate tyrosine kinase receptor signaling. The gene of GIGYF2 has been proposed as the Parkinson-disease (PD) gene underlying the PARK11 locus (PMID: 18358451).