|Positive WB detected in||HEK-293 cells, human spleen tissue, MDCK cells|
|Positive IHC detected in||human testis tissue, mouse kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, MDCK cells, HEK-293 cells|
|Positive FC detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:2000-1:12000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11308-1-AP targets GOLGA2/GM130 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, dog samples.
|Tested Reactivity||human, dog|
|Cited Reactivity||Hamster, human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GOLGA2/GM130 fusion protein Ag1848|
|Full Name||golgi autoantigen, golgin subfamily a, 2|
|Calculated molecular weight||111 kDa|
|Observed molecular weight||130 kDa|
|GenBank accession number||BC014188|
|Gene ID (NCBI)||2801|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and localized mainly at the cytoplasmic face of cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in the regulation of ER-to-Golgi transport and also in the maintenance of the Golgi structure. Emerging evidence suggest that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)
|Product Specific Protocols|
|WB protocol for GOLGA2/GM130 antibody 11308-1-AP||Download protocol|
|IHC protocol for GOLGA2/GM130 antibody 11308-1-AP||Download protocol|
|IF protocol for GOLGA2/GM130 antibody 11308-1-AP||Download protocol|
|FC protocol for GOLGA2/GM130 antibody 11308-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Genetically-encoded FapR-NLuc as a biosensor to determine malonyl-CoA in situ at subcellular scales.
Microglia Express Mu Opioid Receptor: Insights From Transcriptomics and Fluorescent Reporter Mice.
Stem Cells Transl Med
Impaired Bone Regenerative Effect of Exosomes Derived from Bone Marrow Mesenchymal Stem Cells in Type 1 Diabetes.
Antitumor Potential of Extracellular Vesicles Released by Genetically Modified Murine Colon Carcinoma Cells With Overexpression of Interleukin-12 and shRNA for TGF-β1.
Int J Biol Macromol
Exosomal miR-1229 derived from colorectal cancer cells promotes angiogenesis by targeting HIPK2.
J Cell Physiol
Exosomes from human adipose-derived stem cells promote sciatic nerve regeneration via optimizing Schwann cell function.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Stephen (Verified Customer) (10-01-2019)
RPE cells fixed with 4% PFA Perm. by 0.3% tx100 for 5 min blocked with 1% BSA in 1XPBS for 2 hours GM130 antibody (green) incubated 1:300 and EEA1 antibody (purple) overnight at 4 degrees in 1%BSA in 1x PBS. Co-stained with DAPI (blue) visualize DNA