GRK1 Recombinant antibody

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  WB analysis using 85942-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85942-1-RR (GRK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse retina using 85942-1-RR

  mouse retina tissue were subjected to SDS PAGE followed by western blot with 85942-1-RR (GRK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse eye using 85942-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 85942-1-RR (GRK1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse eye using 85942-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 85942-1-RR (GRK1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse eye using 85942-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using GRK1 antibody (85942-1-RR, Clone: 250433A4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 85942-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85942-1-RR against Human GRK1 were performed. The affinity constant is 3.77 nM.

GRK1 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF-P, ELISA

Cat No. 85942-1-RR

Clone No.250433A4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

EC:2.7.11.14, G protein-coupled receptor kinase 1, GPRK1, Rhodopsin kinase, Rhodopsin kinase GRK1

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Tested Applications

• Positive WB detected in: mouse eye tissue, mouse retina tissue
• Positive IHC detected in: mouse eye tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse eye tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:2500-1:10000
• Immunofluorescence (IF)-P: IF-P : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85942-1-RR targets GRK1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GRK1 fusion protein Ag18063
• Full Name: G protein-coupled receptor kinase 1
• Calculated Molecular Weight: 563 aa, 64 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC140945
• Gene Symbol: GRK1
• Gene ID (NCBI): 6011
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q15835
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GRK1(G protein-coupled receptor kinase 1) is also named as RHOK, RK, GPRK1, and belongs to the AGC Ser/Thr protein kinase family. It is involved in quenching of light-induced signal transduction in the phosphoreceptor. GRK1 is a photoreceptor-specific enzyme that mediates adaptation of photoreceptors to light and protects these cells against light-induced injury.