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CoraLite®650-conjugated GS28 Polyclonal antibody

GS28 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite®650 Fluorescent Dye

Cat no : CL650-16106

Synonyms

28 kDa cis Golgi SNARE p28, 28 kDa Golgi SNARE protein, GOLIM2, GOS 28, GOS28, GOS28/P28, GOSR1, GS28, P28

Validation Data Gallery

Applications:
  • FC experiment of HeLa using CL650-16106

    FC experiment of HeLa using CL650-16106

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite®650 Anti-Human GS28 (CL650-16106) (red), or 0.2 ug APC Mouse IgG2b Isotype Control (APC-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 16106-1-AP

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

CL650-16106 targets GS28 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GS28 fusion protein Ag9021
Full Name golgi SNAP receptor complex member 1
Calculated molecular weight28.6 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC012620
Gene symbol GOSR1
Gene ID (NCBI) 9527
Conjugate CoraLite®650 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

GS28, also known as GOSR1, is a 28-kDa Golgi SNARE protein that participates in ER-Golgi and intra-Golgi transport. GS28 is considered to be a core component of the Golgi SNARE complex. (PMID: 8638159)

Protocols

Product Specific Protocols
FC protocol for CL650 GS28 antibody CL650-16106Download protocol
Standard Protocols
Click here to view our Standard Protocols