GSPT2 Recombinant monoclonal antibody

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  WB analysis using 86508-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86508-3-RR (GSPT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 86508-3-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using GSPT2 antibody (86508-3-RR, Clone: 251224C9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 86508-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86508-3-RR against Human GSPT2 were performed. The affinity constant is 2.18 nM.

GSPT2 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 86508-3-RR

Clone No.251224C9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

EC:3.6.5.-, eRF3b, Eukaryotic peptide chain release factor GTP-binding subunit ERF3B, G1 to S phase transition 2, GST2

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Tested Applications

• Positive WB detected in: RKO cells, HEK-293 cells, HepG2 cells, MCF-7 cells, NIH/3T3 cells, C6 cells
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86508-3-RR targets GSPT2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag3641

  Product name: Recombinant human GSPT2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-200 aa of BC036077

  Sequence: MDSGSSSSDSAPDCWDQVDMESTGSAPSGDGVSSAVAEAQREPLSSAFSRKLNVNAKPFVPNVHAAEFVPSFLRGPTQPPTLPAGSGSNDETCTGAGYPQGKRMGRGAPVEPSREEPLVSLEGSNSAVTMELSEPVVENGEVEMALEESWEHSKEVSEAEPGGGSSGDSGPPEESGQEMMEEKEEIRKSKSVIVPSGAPK

• Full Name: G1 to S phase transition 2
• Calculated Molecular Weight: 628 aa, 69 kDa
• Observed Molecular Weight: 69-75 kDa
• GenBank Accession Number: BC036077
• Gene Symbol: GSPT2
• Gene ID (NCBI): 23708
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8IYD1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GSPT2, also named as eukaryotic peptide chain release factor GTP-binding subunit ERF3B or G1 to S phase transition protein 2 homolog, is a 628 amino acid protein, which belongs to the GTP-binding elongation factor family ERF3 subfamily. GSPT2 localizes in the cytoplasm and is highly expressed in IUCC stage II colorectal cancer (CRC). GSPT2 is involved in translation termination in response to the termination codons UAA, UAG, and UGA and may play a role as a potent stimulator of the release factor activity of ETF1. It may play a role in cell cycle progression.