CoraLite®594-conjugated GSTM3 Monoclonal antibody

GSTM3 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E2B8

Cat no : CL594-67634

Synonyms

Glutathione S transferase Mu 3, GST class mu 3, GST5, GSTB, GSTM3, GSTM3 3, GTM3, hGSTM3 3

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Validation Data Gallery

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  IF Staining of A431 using CL594-67634

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite®594 GSTM3 antibody (CL594-67634, Clone: 1E2B8 ) at dilution of 1:200.

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  IF Staining of A431 using CL594-67634

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite®594 GSTM3 antibody (CL594-67634, Clone: 1E2B8 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: A431 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67634 targets GSTM3 in IF applications and shows reactivity with Human, pig samples.

• Tested Reactivity: Human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GSTM3 fusion protein Ag7494
• Full Name: glutathione S-transferase mu 3 (brain)
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC000088
• Gene Symbol: GSTM3
• Gene ID (NCBI): 2947
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GSTM3 is a cytosolic enzyme involved in prostaglandin and leukotriene synthesis and in the metabolization of toxic compounds, such as chemotherapeutic drugs, insecticides, herbicides, carcinogens, and by-products of oxidative stress. GSTM3 is expressed in testis, brain, lung, and lymphocytes. GSTM3 have been reported to be dysregulated in cancers like prostate cancer and colon cancer.