HA Tag Recombinant antibody
HA Tag Recombinant Antibody for WB, IP, IF, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
recombinant protein and More (2)
Applications
WB, IP, IF, ELISA
Conjugate
Unconjugated
CloneNo.
6I21
Cat no : 81290-1-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Transfected HEK-293 cells |
Positive IP detected in | Transfected HEK-293T cells |
Positive IF detected in | Transfected HEK-293 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF) | IF : 1:375-1:1500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 5 publications below |
IF | See 2 publications below |
IP | See 2 publications below |
Product Information
81290-1-RR targets HA Tag in WB, IP, IF, ELISA applications and shows reactivity with recombinant protein samples.
Tested Reactivity | recombinant protein |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Peptide |
Full Name | HA Tag |
Gene Symbol | |
Gene ID (NCBI) | |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for HA Tag antibody 81290-1-RR | Download protocol |
IF protocol for HA Tag antibody 81290-1-RR | Download protocol |
IP protocol for HA Tag antibody 81290-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Cell Biol Manganese regulation of COPII condensation controls circulating lipid homeostasis | ||
Free Radic Biol Med Reprimo (RPRM) mediates neuronal ferroptosis via CREB-Nrf2/SCD1 pathways in radiation-induced brain injury | ||
Mol Nutr Food Res Ethanol Extract of Citrus grandis 'Tomentosa' Exerts Anticancer Effects by Targeting Skp2/p27 Pathway in Non-Small Cell Lung Cancer | ||
Res Sq VprBP/DCAF1 triggers melanomagenic gene silencing through histone H2A phosphorylation | ||
Int J Biol Sci Hypoxia-driven TNS4 fosters HNSCC tumorigenesis by stabilizing integrin α5β1 complex and triggering FAK-mediated Akt and TGFβ signaling pathways | ||
Cell Mol Life Sci BCLAF1 binds SPOP to stabilize PD-L1 and promotes the development and immune escape of hepatocellular carcinoma |