Tested Applications
| Positive WB detected in | Transfected HEK-293 cells |
| Positive IP detected in | Transfected HEK-293T cells |
| Positive IF/ICC detected in | Transfected HEK-293 cells |
| Positive FC (Intra) detected in | Transfected HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
81290-1-RR targets HA Tag in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Cited Reactivity | human, mouse, chicken, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | HA Tag |
| Calculated Molecular Weight | 1 kDa |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2935602 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | HATAG |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for HA Tag antibody 81290-1-RR | Download protocol |
| IF protocol for HA Tag antibody 81290-1-RR | Download protocol |
| IP protocol for HA Tag antibody 81290-1-RR | Download protocol |
| WB protocol for HA Tag antibody 81290-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The HA Tag Recombinant monoclonal antibody (6I21), catalog number 81290-1-RR, has accumulated 150 citations. Publications appear in high-impact journals including Nature Metabolism, Nature Cell Biology, Nature Communications, Molecular Cell, Science Translational Medicine, Advanced Science, Cell Death & Differentiation, and Theranostics, among others. Research fields span oncology, ferroptosis, ubiquitin-proteasome system, virology, cardiology, immunology, neuroscience, metabolism, autophagy, and signaling pathway research.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther DNA/RNA-binding protein KIN17 supports esophageal cancer progression via resolving noncanonical STING activation induced by R-loop. | ||
Nat Metab Oligodendrocyte precursor cell-specific blocking of low-glucose-induced activation of AMPK ensures myelination and remyelination | ||
Nat Cell Biol Manganese regulation of COPII condensation controls circulating lipid homeostasis | ||
Nat Cell Biol Defining heterogeneity in core regulatory circuitry reveals HOXB3 condensation as a potential target in glioblastoma. | ||
Drug Resist Updat Lactate-driven H3K18 lactylation promotes cisplatin resistance in bladder cancer via HNRNPF-Parkin mediated mitophagy. | ||
Nat Commun Optimized in vivo base editing restores auditory function in a DFNA15 mouse model |
Reviews
What customers say
User feedback is mixed. One reviewer (5/5) praised it as the best HA antibody they've used, reporting crisp, specific staining with minimal background in IF on U2OS cells at 1:500 dilution, consistent across both stable and transiently transfected lines. Another reviewer (1/5) reported unreliable signal in their positive cell line and notable nuclear background in both positive and negative controls during IF on iPSC-derived cardiomyocytes at 1:400 dilution. Results appear to vary by cell type and experimental setup.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Kiara (Verified Customer) (07-28-2025) | Did not give a reliable signal in our positive cell line (CRISPRi that should have HA-tagged dCas9-KRAB). Additionally, there is quite some background as in both the positive and the negative line (WTC11), the nuclei are stained.
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Emile (Verified Customer) (04-08-2025) | This HA Tag Recombinant antibody (81290-1-RR) is by far the best HA antibody I have used. It worked exceptionally well in my hands for immunofluorescence experiments. I tested it on a stable cell line expressing an HA-tagged protein and on cells transiently transfected with an HA-tagged protein, and the results were consistently outstanding. The staining was crisp and specific, with minimal background. I used it at a 1:500 dilution on U2OS cells and achieved excellent results.
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