Tested Applications
| Positive WB detected in | Transfected HEK-293 cells |
| Positive IP detected in | Transfected HEK-293T cells |
| Positive IF/ICC detected in | Transfected HEK-293 cells |
| Positive FC (Intra) detected in | Transfected HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
81290-1-RR targets HA Tag in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Cited Reactivity | human, mouse, chicken, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | HA Tag |
| Calculated Molecular Weight | 1 kDa |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2935602 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | HATAG |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for HA Tag antibody 81290-1-RR | Download protocol |
| IF protocol for HA Tag antibody 81290-1-RR | Download protocol |
| IP protocol for HA Tag antibody 81290-1-RR | Download protocol |
| WB protocol for HA Tag antibody 81290-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
HA Tag Recombinant monoclonal antibody (6I21), catalog number 81290-1-RR, has 131 citations. These appear in high-impact journals including Nature Communications, Nature Cell Biology, Molecular Cell, Cell Reports, Cell Death & Differentiation, Advanced Science, Theranostics, and Journal of Clinical Investigation. Research fields span cancer biology, ferroptosis, ubiquitination, cardiac disease, neurodegeneration, virology, immune signaling, metabolism, stem cell biology, and veterinary medicine.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther DNA/RNA-binding protein KIN17 supports esophageal cancer progression via resolving noncanonical STING activation induced by R-loop. | ||
Nat Metab Oligodendrocyte precursor cell-specific blocking of low-glucose-induced activation of AMPK ensures myelination and remyelination | ||
Nat Cell Biol Manganese regulation of COPII condensation controls circulating lipid homeostasis | ||
Nat Cell Biol Defining heterogeneity in core regulatory circuitry reveals HOXB3 condensation as a potential target in glioblastoma. | ||
Drug Resist Updat Lactate-driven H3K18 lactylation promotes cisplatin resistance in bladder cancer via HNRNPF-Parkin mediated mitophagy. | ||
Nat Commun Lack of intestinal Mucin-1 impairs intestinal epithelial barrier and promotes metabolic dysfunction-associated steatotic liver disease in male mice. |
Reviews
What customers say
Reviews for this HA Tag Recombinant antibody are mixed. One user reported excellent performance in immunofluorescence on U2OS cells at 1:500 dilution, describing crisp, specific staining with minimal background on both stable and transiently transfected HA-tagged cell lines. Another user, however, rated it poorly, noting unreliable signal in a CRISPRi HA-tagged dCas9-KRAB positive cell line and significant nuclear background in both positive and negative iPSC-derived cardiomyocytes at 1:400 dilution. Overall, results appear highly application- and cell-type-dependent.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Kiara (Verified Customer) (07-28-2025) | Did not give a reliable signal in our positive cell line (CRISPRi that should have HA-tagged dCas9-KRAB). Additionally, there is quite some background as in both the positive and the negative line (WTC11), the nuclei are stained.
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Emile (Verified Customer) (04-08-2025) | This HA Tag Recombinant antibody (81290-1-RR) is by far the best HA antibody I have used. It worked exceptionally well in my hands for immunofluorescence experiments. I tested it on a stable cell line expressing an HA-tagged protein and on cells transiently transfected with an HA-tagged protein, and the results were consistently outstanding. The staining was crisp and specific, with minimal background. I used it at a 1:500 dilution on U2OS cells and achieved excellent results.
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