Tested Applications
| Positive WB detected in | Transfected HEK-293T cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
HRP-81290 targets HA Tag in WB, IP, CoIP applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | HA Tag |
| Calculated Molecular Weight | 1 kDa |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_3086578 |
| Conjugate | HRP |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | HATAG |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.
Publications
What published studies show
HRP-81290 is the HRP-conjugated HA Tag Recombinant monoclonal antibody (6I21). It has 17 citations published in journals including Theranostics, Cell Death & Disease, Oncogene, Cell Reports, Journal of Medicinal Chemistry, International Journal of Molecular Sciences, PLoS Pathogens, Journal of Virology, PLoS Genetics, PLoS One, Marine Drugs, Frontiers in Cellular Neuroscience, and Heliyon. Research fields span oncology, virology, immunology, epigenetics, and circadian biology.
| Species | Application | Title |
|---|---|---|
Theranostics USP14 modulates stem-like properties, tumorigenicity, and radiotherapy resistance in glioblastoma stem cells through stabilization of MST4-phosphorylated ALKBH5 | ||
Cell Death Dis USP3 promotes DNA damage response and chemotherapy resistance through stabilizing and deubiquitinating SMARCA5 in prostate cancer | ||
Cell Death Dis AKT1 phosphorylates PRMT7 to promote GLUD1 methylation and gastric cancer progression. | ||
Oncogene MST4 plays dual roles in lung adenocarcinoma by regulating homeostasis of wild-type and mutant p53 protein. | ||
Cell Rep A pro-metastatic tRNA fragment drives aldolase A oligomerization to enhance aerobic glycolysis in lung adenocarcinoma | ||
Reviews
What customers say
One reviewer tested this antibody by Western blot on transfected HeLa cells expressing an HA-tagged protein. It performed very well at a 1/5000 dilution overnight, earning a 5-star rating. The user reported clear and reliable results with no issues mentioned.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Margaux (Verified Customer) (11-04-2025) | I tested this antibody by western-blot on HeLa cells which were transfected with a plasmid to express a HA-tagged protein. It worked very well at the dilution 1/5000 overnight.
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