Tested Applications
| Positive WB detected in | Transfected HEK-293T cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
HRP-81290 targets HA Tag in WB, IP, CoIP applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | HA Tag |
| Calculated Molecular Weight | 1 kDa |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_3086578 |
| Conjugate | HRP |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | HATAG |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.
Publications
What published studies show
HRP-81290 is the HA Tag Recombinant monoclonal antibody (6I21), with 17 citations. These appear in journals including Theranostics, Cell Death & Disease, Oncogene, Cell Reports, J Med Chem, Marine Drugs, Int J Mol Sci, PLoS Pathogens, Front Cell Neurosci, J Virol, PLoS Genetics, PLoS One, and Heliyon. Research fields span oncology (glioblastoma, gastric, prostate, lung, and pancreatic cancers), virology (monkeypox, PEDV, coronavirus), neurodevelopmental disorders, circadian biology, DNA damage response, and immune signaling.
| Species | Application | Title |
|---|---|---|
Theranostics USP14 modulates stem-like properties, tumorigenicity, and radiotherapy resistance in glioblastoma stem cells through stabilization of MST4-phosphorylated ALKBH5 | ||
Cell Death Dis USP3 promotes DNA damage response and chemotherapy resistance through stabilizing and deubiquitinating SMARCA5 in prostate cancer | ||
Cell Death Dis AKT1 phosphorylates PRMT7 to promote GLUD1 methylation and gastric cancer progression. | ||
Oncogene MST4 plays dual roles in lung adenocarcinoma by regulating homeostasis of wild-type and mutant p53 protein. | ||
Cell Rep A pro-metastatic tRNA fragment drives aldolase A oligomerization to enhance aerobic glycolysis in lung adenocarcinoma |
Reviews
What customers say
The single review for HRP-81290 is a 5-star rating from a Western Blot application. The user tested the antibody on transfected HeLa cells expressing an HA-tagged protein and reported it performed very well at a 1/5000 dilution with an overnight incubation. Overall, the feedback is positive, highlighting reliable performance and strong signal in WB.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Margaux (Verified Customer) (11-04-2025) | I tested this antibody by western-blot on HeLa cells which were transfected with a plasmid to express a HA-tagged protein. It worked very well at the dilution 1/5000 overnight.
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