HBB Polyclonal antibody, PBS Only

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• HBB Polyclonal antibody, PBS Only (16216-1-PBS)

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  WB analysis of human heart using 16216-1-AP (same clone as 16216-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 16216-1-AP (HBB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16216-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 16216-1-AP (same clone as 16216-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 16216-1-AP (HBB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16216-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse heart using 16216-1-AP (same clone as 16216-1-PBS)

  IP result of anti-HBB (IP:16216-1-AP, 4ug; Detection:16216-1-AP 1:1000) with mouse heart tissue lysate 3200ug. This data was developed using the same antibody clone with 16216-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 16216-1-AP (same clone as 16216-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 16216-1-AP (HBB antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16216-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 16216-1-AP (same clone as 16216-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 16216-1-AP (HBB antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16216-1-PBS in a different storage buffer formulation.

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  IF Staining of human appendicitis using 16216-1-AP (same clone as 16216-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using HBB antibody (16216-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16216-1-PBS in a different storage buffer formulation.

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  IF Staining of human placenta using 16216-1-AP (same clone as 16216-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using HBB antibody (16216-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16216-1-PBS in a different storage buffer formulation.

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  FC experiment of K-562 using 16216-1-AP (same clone as 16216-1-PBS)

  1X10^6 K-562 cells were intracellularly stained with 0.8 ug Anti-Human HBB (16216-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 16216-1-PBS in a different storage buffer formulation.

HBB Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA

Cat No. 16216-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Synonyms

Spinorphin, LVV-hemorphin-7, Hemoglobin subunit beta, Hemoglobin beta chain, Beta-globin

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Product Information

16216-1-PBS targets HBB in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9216

  Product name: Recombinant human HBB protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-147 aa of BC007075

  Sequence: MVHLTPEEKSAVTALWGKVNVDEVGGEALGRLLVVYPWTQRFFESFGDLSTPDAVMGNPKVKAHGKKVLGAFSDGLAHLDNLKGTFATLSELHCDKLHVDPENFRLLGNVLVCVLAHHFGKEFTPPVQAAYQKVVAGVANALAHKYH

• Full Name: hemoglobin, beta
• Calculated Molecular Weight: 147 aa, 16 kDa
• Observed Molecular Weight: 13-16 kDa
• GenBank Accession Number: BC007075
• Gene Symbol: HBB
• Gene ID (NCBI): 3043
• RRID: AB_10598329
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P68871
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBB gene encodes hemoglobin beta chain. The hemoglobin chains, each with its own heme moiety, cooperate in binding and release of oxygen. Hemoglobin monomers have been found in blood and other tissues including macrophages, alveolar epithelial cells and brain. Defects in HBB have been linked to diseases including Sickle cell anemia, Beta-thalassemia, and Heinz body anemias. This antibody, raised against the full-length human HBB, recognizes the 13-16 kDa HBB in western blot analyses of human heart and placenta.