HINT1 Monoclonal antibody
HINT1 Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
1G3E4
Cat no : 67583-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | LNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
Product Information
67583-1-Ig targets HINT1 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | HINT1 fusion protein Ag16662 |
Full Name | histidine triad nucleotide binding protein 1 |
Calculated molecular weight | 14 kDa |
Observed molecular weight | 14 kDa |
GenBank accession number | BC007090 |
Gene symbol | HINT1 |
Gene ID (NCBI) | 3094 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HINT1(Histidine triad nucleotide-binding protein 1) is also named as HINT, PKCI1, PRKCNH1,which is a member of the histidine triad (HIT) family, highly conserved in diverse species and ubiquitously expressed in mammalian tissues.It hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2.
Protocols
Product Specific Protocols | |
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WB protocol for HINT1 antibody 67583-1-Ig | Download protocol |
IHC protocol for HINT1 antibody 67583-1-Ig | Download protocol |
IF protocol for HINT1 antibody 67583-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |