Anti-Human HLA-DR (L243)

HLA-DR Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

L243

Cat no : 65218-1-Ig

Synonyms

HLA DRA, HLA DRB, HLA-DR, HLA-DRA, HLA-DRB, MHC class II antigen DRA

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Validation Data Gallery

• 

  FC experiment of human PBMCs using 65218-1-Ig

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human HLA-DR (65218-1-Ig, Clone:L243) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using 65218-1-Ig

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human HLA-DR (65218-1-Ig, Clone:L243) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Monocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65218-1-Ig targets HLA-DR in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Human lymphoblastoid B-cell line RPMI 8866
• Full Name: major histocompatibility complex, class II, DR alpha
• Calculated Molecular Weight: 254 aa, 29 kDa
• GenBank Accession Number: BC032350
• Gene Symbol: HLA-DRA
• Gene ID (NCBI): 3122
• RRID: AB_2918474
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.