Anti-Human HLA-DR (L243)

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• Anti-Human HLA-DR (L243) (65218-1-Ig)

HLA-DR Monoclonal Antibody for FC

Cat No. 65218-1-Ig Copied!

Clone No. L243

Publications (5)

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Host / Isotype

Mouse / IgG2a, kappa

Reactivity

human, cynomolgus monkey

Applications

IF, FC

Synonyms

HLA-DRA, HLA DRA, HLA DRA1, HLA DRB, HLA-DRA1

• 

  FC experiment of human PBMCs using 65218-1-Ig

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human HLA-DR (65218-1-Ig, Clone:L243) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Monocytes were gated.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of human PBMCs using 65218-1-Ig

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human HLA-DR (65218-1-Ig, Clone:L243) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Monocytes were gated.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.2 ug per 10^6 cells in 100 ul suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

65218-1-Ig targets HLA-DR in IF, FC applications and shows reactivity with human, cynomolgus monkey samples.

• Tested Reactivity: human, cynomolgus monkey
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Human lymphoblastoid B-cell line RPMI 8866

• Full Name: major histocompatibility complex, class II, DR alpha
• Calculated Molecular Weight: 254 aa, 29 kDa
• GenBank Accession Number: BC032350
• Gene Symbol: HLA-DRA
• Gene ID (NCBI): 3122
• ENSEMBL Gene ID: ENSG00000204287
• RRID: AB_2918474
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P01903
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Publications

What published studies show

Anti-Human HLA-DR (L243), catalog number 65218-1-Ig, has 5 published citations. These appear in Advanced Science, International Journal of Biological Sciences, Journal of Neuroinflammation, Advanced Wound Care, and Cell Oncology. The research fields span tumor immunology (nasopharyngeal carcinoma, urothelial carcinoma, osteosarcoma), neuroinflammation (astrocytic antigen presentation), and stem cell biology (wound healing). Applications cited include flow cytometry and immunofluorescence in human samples.

Automatically generated from the 5 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	FC	Adv Sci (Weinh); CD20(+)FCRL4(+) B Cells Activate CD8(+) T Cells via MHC-I Restriction in Nasopharyngeal Carcinoma Anti-Tumor Immunity.; Authors - Benjian Zhang
human	IF	Int J Biol Sci; Single-cell and spatial transcriptomic analysis reveal cellular heterogeneity and cancer cell-intrinsic major histocompatibility complex II expression in urothelial carcinoma.; Authors - Shengwei Xiong
human	IF	J Neuroinflammation; Astrocytic lipid droplets contain MHCII and may act as cogs in the antigen presentation machinery; Authors - Chiara Beretta
human	FC	Adv Wound Care (New Rochelle); Engineered Mesenchymal Stem Cells with Endogenous Trehalose Expression Activate the NRF2-HMOX1 Pathway to Enhance Antioxidant Stress and Wound Healing Capacity.; Authors - Zhen Wang
		Cell Oncol (Dordr); Machine learning-based integrative analysis identifies CXCL13-driven tertiary lymphoid structures as favorable immune and prognostic features in osteosarcoma.; Authors - Jie Jiang